1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. CAPN2 蛋白, Human (His)

CAPN2 蛋白, Human (His)

目录号: HY-P72115
COA 产品使用指南

CAPN2 (Calpain-2) 是一种钙调节的非溶酶体硫醇蛋白酶,因催化对细胞骨架重塑和信号转导至关重要的底物的有限蛋白水解而闻名。值得注意的是,CAPN2 在“Arg-226”处以蛋白水解方式裂解 MYOC,表明它参与调节该蛋白质的功能。CAPN2 蛋白, Human (His) 是重组的 CAPN2 蛋白,由 E. coli 表达,带有 N-6*His 标签。CAPN2 蛋白, Human (His) 全长 681 个氨基酸,分子量约为 ~82.1 kDa。

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  • 生物活性

  • 技术参数

  • 产品性质

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描述

CAPN2 (Calpain-2) is a calcium-regulated, non-lysosomal thiol protease known for catalyzing the limited proteolysis of substrates critical for cytoskeletal remodeling and signaling. Notably, CAPN2 proteolytically cleaves MYOC at “Arg-226,” suggesting its involvement in regulating the function of this protein. CAPN2 Protein, Human (His) is the recombinant human-derived CAPN2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of CAPN2 Protein, Human (His) is 681 a.a., with molecular weight of ~82.1 kDa.

研究背景

CAPN2 是一种钙调节的非溶酶体硫醇蛋白酶,在参与细胞骨架重塑和信号转导的底物有限蛋白水解中充当关键酶。CAPN2 以钙依赖性方式运作,催化靶向蛋白水解,其在特定位点“Arg-226”切割 MYOC 就证明了这一点。此外,CAPN2 参与神经元刺激诱导的 CPEB3 蛋白水解裂解的调节,导致 CPEB3 翻译抑制活性的消除。该事件随后促进了 CPEB3 靶标 mRNA 的翻译,突出了 CAPN2 在协调与细胞骨架动力学、信号转导和神经元功能相关的细胞过程中的多方面作用。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P17655 (S20-L700)

基因 ID

824  [NCBI]

蛋白结构
N-term
6*His
CAPN2 (S20-L700)
Accession # P17655
C-term
同用名
Calcium-activated neutral proteinase 2; Calpain M-type; Millimolar-calpain; CAPN2; CANPL2
氨基酸序列

SHDRAIKYLNQDYEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSDFLRHYSRLEICNLTPDTLTSDTYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELSGQTNIHLSKNFFLTNRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKDGDFCIRVFSEKKADYQAVDDEIEANLEEFDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIELDLISWLCFSVL

分子量

Approximately 82.1 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

CAPN2 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CAPN2 Protein, Human (His)
目录号:
HY-P72115
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