Cardiac phospholamban/PLN, Human (P.pastoris, GST)

Cardiac phospholamban (PLN) strictly regulates ATP2A2 in the cardiac sarcoplasmic reticulum, reversibly inhibits ATPase activity and affects myocardial contractility. Modulation of PLN reduces the affinity of ATPase for Ca(2+), thereby affecting calcium reuptake during muscle relaxation and maintaining cardiac calcium homeostasis. Cardiac phospholamban/PLN, Human (P.pastoris, GST) is the recombinant human-derived Cardiac phospholamban/PLN, expressed by P. pastoris , with N-GST labeled tag. The total length of Cardiac phospholamban/PLN, Human (P.pastoris, GST) is 52 a.a., with molecular weight of ~33.1 kDa.

心脏受磷蛋白 (PLN) 是一种关键调节剂，通过降低 ATP 酶对 Ca(2+) 的表观亲和力，可逆地抑制心脏肌浆网中 ATP2A2 的活性。PLN 对 ATP2A2 的调节显着影响心肌响应生理刺激的收缩性，影响肌肉松弛过程中钙的再摄取，并在维持心肌内钙稳态中发挥关键作用。ATP2A2 抑制的程度取决于 PLN 的寡聚状态，PLN 的磷酸化可减轻 ATP2A2 的抑制。PLN 还可以控制细长精子细胞中的细胞内 Ca(2+) 水平，表明其在生殖细胞分化中具有潜在作用。作为同五聚体，PLN 与多种蛋白质相互作用，包括 HAX1、ATP2A2、VMP1 和 S100A1，有助于其在细胞过程中的多方面调节功能。

Human

P. pastoris

N-GST

P26678 (M1-L52)

5350  [NCBI]

MEKVQYLTRSAIRRASTIEMPQQARQKLQNLFINFCLILICLLLICIIVMLL

Approximately 33.1kDa

• 
  Greater than 97% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 3% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.