CBLB/RNF56 蛋白, Human (HEK293, His)

The CBLB/RNF56 protein is an E3 ubiquitin protein ligase that coordinates substrate degradation through ubiquitin transfer by specific E2 enzymes. It crucially inhibits signal transduction pathways initiated by TCR, BCR and FCER1. CBLB/RNF56 Protein, Human (HEK293, His) is the recombinant human-derived CBLB/RNF56 protein, expressed by HEK293 , with N-His labeled tag. The total length of CBLB/RNF56 Protein, Human (HEK293, His) is 388 a.a..

CBLB/RNF56 蛋白作为 E3 泛素蛋白连接酶，介导泛素从特定 E2 泛素结合酶转移至底物，最终促进蛋白酶体降解。它在 TCR、BCR 和 FCER1 启动的信号转导通路负调节中发挥着关键作用。在初始 T 细胞中，CBLB 在 TCR 参与时抑制 VAV1 激活，并要求 CD28 共刺激以促进增殖和 IL-2 产生。此外，它还能促进 PIK3R1/p85 泛素化，阻碍其招募至 TCR 并随后激活。在激活的 T 细胞中，CBLB 会阻碍 PLCG1 的激活和再刺激时的钙动员，从而导致无反应性的诱导。在 B 细胞中，它泛素化 SYK，促进其蛋白酶体降解。此外，CBLB 可能在 SRC 泛素化中发挥作用，并可能参与 EGFR 泛素化和内化。建议与 E2 泛素蛋白连接酶 UB2D3 结合使用，当与 CBL 结合时，对于通过 PDGFRA 泛素化和内化来正确反馈抑制睫状 PDGFRA 信号传导至关重要。

Human

HEK293

N-6*His

Q13191-1 (A39-F426)

868  [NCBI]

AAADRRTVEKTWKLMDKVVRLCQNPKLQLKNSPPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDRRNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKPGSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPHDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCEIKGTEPIIVDPF

Approximately 44-46 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of 40 mM HEPES, 120 mM NaCl, pH 6.8, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.