CBR3 蛋白, Human (His)

The CBR3 protein uses NADPH to convert carbonyl compounds into alcohols. CBR3 Protein, Human (His) is the recombinant human-derived CBR3 protein, expressed by E. coli , with N-His labeled tag. The total length of CBR3 Protein, Human (His) is 277 a.a., with molecular weight of ~37 kDa.

CBR3 是一种多面酶，可催化各种羰基化合物的 NADPH 依赖性还原，将它们转化为各自的醇。尽管 CBR3 表现出较低的 NADPH 依赖性氧化还原酶活性，但它对邻醌（包括 1,2-萘醌）特别有效，显示出其在防止外源醌的细胞毒性作用方面的潜在作用。该酶不仅限于醌类化合物，它还作用于非醌类化合物，如靛红和抗癌药物 oracin。CBR3 对邻醌而非对醌的偏好凸显了其底物特异性。值得注意的是，除了靛红之外，尚未发现 CBR3 的内源性底物，这强调了它的多功能性和对细胞解毒过程的潜在贡献。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-His

O75828 (M1-W277)

874  [NCBI]

MSSCSRVALVTGANRGIGLAIARELCRQFSGDVVLTARDVARGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLNVLVNNAAVAFKSDDPMPFDIKAEMTLKTNFFATRNMCNELLPIMKPHGRVVNISSLQCLRAFENCSEDLQERFHSETLTEGDLVDLMKKFVEDTKNEVHEREGWPNSPYGVSKLGVTVLSRILARRLDEKRKADRILVNACCPGPVKTDMDGKDSIRTVEEGAETPVYLALLPPDATEPQGQLVHDKVVQNW

Approximately 37 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.