TECK/CCL25 蛋白, Mouse

The CCL25 protein emerged as a potential coordinator of T cell development, suggesting its involvement in the complex process of T cell maturation. Its recombinant form exhibits chemotactic activity against various immune cell types, including thymocytes and macrophages, by binding to the chemokine receptor CCR9. TECK/CCL25 Protein, Mouse is the recombinant mouse-derived TECK/CCL25 protein, expressed by E. coli , with tag free.

CCL25 蛋白作为 T 细胞发育的潜在参与者而出现，表明它在协调与 T 细胞成熟相关的复杂过程中发挥着作用。CCL25 的重组形式对各种免疫细胞类型（包括胸腺细胞、巨噬细胞、THP-1 细胞和树突状细胞）显示出趋化活性，同时对外周血淋巴细胞和中性粒细胞保持无活性。该蛋白通过与趋化因子受体 CCR9 结合来发挥作用，表明它参与指导特定细胞群的迁移。此外，CCL25 与非典型趋化因子受体 ACKR4 结合，促进 β-arrestin (ARRB1/2) 募集到 ACKR4。这种多样化的相互作用强调了 CCL25 在免疫细胞运输中的潜在调节作用，并表明它在协调免疫反应和 T 细胞相关过程中的重要性。

Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR9 .The ED₅₀ for this effect is 0.1269μg/mL, corresponding to a specific activity is 7880.221 U/mg.

Mouse

E. coli

Tag Free

O35903 (Q24-N144)

20300  [NCBI]

QGAFEDCCLGYQHRIKWNVLRHARNYHQQEVSGSCNLRAVRFYFRQKVVCGNPEDMNVKRAIRILTARKRLVHWKSASDSQTERKKSNHMKSKVENPNSTSVRSATLGHPRMVMMPRKTNN

Predict MW: 14.15 kDa; Approximately 16 kDa band in SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in sterile distilled water.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.