1. 重组蛋白
  2. CD Antigens
  3. NK Cell CD Proteins
  4. CD100/Semaphorin-4D
  5. Semaphorin-4D/SEMA4D 蛋白, Human (HEK293, His)

Semaphorin-4D/SEMA4D 蛋白, Human (HEK293, His)

目录号: HY-P7774
COA 产品使用指南

CD100 蛋白, Human (HEK293, His)是信号量家族的一种蛋白质,在血管,神经和免疫系统中起重要作用。

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规格 价格 是否有货 数量
10 μg ¥1050 In-stock
50 μg ¥3142 In-stock
100 μg   询价  

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  • 参考文献

描述

Semaphorin-4D/SEMA4D Protein, Human (HEK293, His) is a protein from the semaphorin family with important roles in the vascular, nervous and immune systems.

研究背景

Semaphorin 4D、Sema4D 或 CD100 属于 4 类信号蛋白,是所谓的“免疫信号蛋白”之一。CD100 由大多数造血细胞(包括 B 和 T 淋巴细胞、自然杀伤细胞和骨髓细胞以及内皮细胞)产生,根据细胞类型和生物体,通过与不同受体结合来发挥作用。除了在膜中表达外,CD100 还以可溶形式 (sCD100) 存在,它是通过蛋白水解裂解以激活依赖性方式从膜 CD100 (mCD100) 产生的[1]

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q92854-1 (M22-R734)

基因 ID
蛋白结构
N-term
SEMA4D (M22-R734)
Accession # Q92854-1
6*His
C-term
中文名
重组人CD100, His (HEK293 细胞表达)
同用名
rHuCD100, His; Semaphorin-4D; A8; BB18; GR3; CD100
氨基酸序列

MAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNRHHHHHH

分子量

Approximately 112 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Semaphorin-4D/SEMA4D Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Semaphorin-4D/SEMA4D Protein, Human (HEK293, His)
目录号:
HY-P7774
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