CD3 gamma 蛋白, Human (HEK293, Fc)

CD3 gamma, part of the TCR-CD3 complex on T-lymphocytes, facilitates TCR-mediated signaling and regulates surface expression. APC-induced TCR activation prompts CD3 gamma, along with CD3D, CD3E, and CD3Z, to transmit signals via ITAMs. Phosphorylation by LCK and FYN initiates downstream signaling. CD3 gamma's diL motif guides constitutive TCR cycling. In TCR-CD3 assembly, CD3 gamma shows versatility, forming heterodimers and trimers, highlighting its pivotal role in T-cell activation and receptor dynamics. CD3 gamma Protein, Human (HEK293, Fc) is the recombinant human-derived CD3 gamma protein, expressed by HEK293, with C-hFc labeled tag. The total length of CD3 gamma Protein, Human (HEK293, Fc) is 94 a.a., with molecular weight of 45-55 kDa.

CD3 γ 是 T 淋巴细胞表面 TCR-CD3 复合物的组成部分，在适应性免疫反应中至关重要。当抗原呈递细胞 (APC) 激活 T 细胞受体 (TCR) 时，CD3 γ 与其他 CD3 链（CD3D、CD3E 和 CD3Z）一起促进 TCR 介导的信号跨细胞膜的传输。CD3 γ 的胞质结构域中存在基于免疫受体酪氨酸的激活基序 (ITAM)，在 TCR 参与后，CD3 γ 会被 Src 家族蛋白酪氨酸激酶 LCK 和 FYN 磷酸化，从而激活下游信号通路。除了在信号转导中的作用之外，CD3 γ 在动态调节细胞表面 TCR 表达方面也发挥着至关重要的作用。组成型 TCR 循环依赖于 CD3 γ 中存在的基于二亮氨酸 (diL) 的受体分选基序。TCR-CD3复合物包含CD3D/CD3E和CD3G/CD3E异二聚体，其优先与TCRα和TCRβ缔合，形成与CD3Z同二聚体相互作用的三聚体以完成六聚体TCR-CD3复合物。或者，TCRalpha 和 TCRbeta 可以替换为 TCRgamma 和 TCRdelta，展示 CD3 gamma 在 TCR 组装和功能方面的多功能性。

Human

HEK293

C-hFc

P09693 (Q23-S116)

917  [NCBI]

45-55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.