1. 重组蛋白
  2. CD Antigens
  3. Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins
  4. CD300c
  5. CD300c 蛋白, Human (HEK293, His)

CD300c 蛋白, Human (HEK293, His)

目录号: HY-P76222
COA 产品使用指南

CD300c 蛋白,也称为 CMRF35 抗原,使用单克隆抗体进行鉴定。正如 Jackson 等人所记录的,它存在于单核细胞、中性粒细胞以及某些 T 和 B 淋巴细胞等免疫细胞上。1992 年。该蛋白广泛表达于多种组织中,包括肺、阑尾和其他 19 种组织。CD300c 蛋白, Human (HEK293, His) 是重组的 CD300c 蛋白,由 HEK293 表达,带有 C-His 标签。

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规格 价格 是否有货 数量
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5 μg ¥450 In-stock
10 μg ¥750 In-stock
50 μg ¥2100 In-stock
100 μg ¥3600 In-stock
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CD300c protein, also known as CMRF35 antigen, was identified using a monoclonal antibody. It is present on immune cells like monocytes, neutrophils, and certain T and B lymphocytes, as documented by Jackson et al. in 1992. The protein is broadly expressed in various tissues, including the lung, appendix, and 19 other tissues. CD300c Protein, Human (HEK293, His) is the recombinant human-derived CD300c protein, expressed by HEK293 , with C-His labeled tag.

研究背景

CD300c 蛋白,也称为 CMRF35 抗原,已通过与单克隆抗体的反应来鉴定。Jackson 等人的一项研究记录了这种抗原存在于各种免疫细胞上,包括单核细胞、中性粒细胞以及某些 T 和 B 淋巴细胞。1992 年。该蛋白在多种组织中广泛表达,尤其在肺、阑尾和其他 19 种组织中明显存在。

生物活性

Measured by its ability to inhibit anti-CD3-induced proliferation of Jurkat human T-lymphocyte leukemia cells. The ED50 this effect is 3.884 μg/ml, corresponding to a specific activity is 257.47 units/mg.

  • Measured by its ability to inhibit anti-CD3-induced proliferation of Jurkat human T-lymphocyte leukemia cells. The ED50 this effect is 3.884 μg/mL, corresponding to a specific activity is 257.47 units/mg.
种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

Q08708/NP_006669.1 (M29-R183)

基因 ID
蛋白结构
N-term
CD300c (M29-R183)
Accession # Q08708/NP_006669.1
His
C-term
同用名
CMRF35-like molecule 6; CLM-6; CMRF35-A1; CMRF-35; IGSF16
分子量

29-40 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD300c Protein, Human (HEK293, His)
目录号:
HY-P76222
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