Fc gamma RIIA/CD32a 蛋白, Human (HEK293, His)

Fc gamma The RIIA/CD32a protein plays a key role as a low-affinity receptor that specifically binds to the Fc region of immunoglobulin gamma. It interacts with IgG to initiate cellular responses against pathogens, which is critical for immune regulation. Fc gamma RIIA/CD32a Protein, Human (HEK293, His) is the recombinant human-derived Fc gamma RIIA/CD32a protein, expressed by HEK293 , with C-6*His labeled tag and H167, , , , mutation.

Fc γ RIIA/CD32a 蛋白通过特异性结合免疫球蛋白 γ 的 Fc 区而发挥关键作用，充当低亲和力受体。通过与 IgG 相互作用，Fc gamma RIIA/CD32a 启动针对病原体和可溶性抗原的细胞反应，说明其在免疫调节中的重要作用。值得注意的是，该蛋白质促进调理抗原的吞噬作用，进一步促进免疫防御机制。此外，Fc gamma RIIA/CD32a 与 IGHG1、INPP5D/SHIP1、INPPL1/SHIP2、APCS、FGR 和 HCK 相互作用，表明其参与复杂的信号传导途径及其细胞功能的调节。这些多方面的相互作用强调了 Fc gamma RIIA/CD32a 在协调不同免疫反应中的重要性。

1. Loaded Human CD32a His on HIS1K Biosensor, can bind Anti-Human HER2 mAb with an affinity constant of 0.98 uM as determined in BLI assay.
2. Immobilized  Human CD32a at 10 μg/mL (100 μL/well) can bind Biotinylated Human IgG1  protein. The ED₅₀ for this effect is 3.612 μg/mL, corresponding to a specific  activity is 2.77×10² Unit/mg.

• 
  Immobilized Human CD32a at 10 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 protein. The ED₅₀ for this effect is 3.612 μg/mL, corresponding to a specific activity is 2.77×10² Unit/mg.

Human

HEK293

C-6*His

P12318-1 (A36-I218, H167)

2212  [NCBI]

AAPPKAVLKLEPPWINVLQEDSVTLTCQGARSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIMLRCHSWKDKPLVKVTFFQNGKSQKFSHLDPTFSIPQANHSHSGDYHCTGNIGYTLFSSKPVTITVQVPSMGSSSPMGI

25-34 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.