2. 重组蛋白
  3. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens
  4. TNF Superfamily Stimulatory Immune Checkpoint Molecules T Cell CD Proteins B Cell CD Proteins Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  5. TNF Receptor Superfamily CD40 GITR/CD357
  6. CD40 蛋白, Mouse (HEK293, His)

CD40 蛋白, Mouse (HEK293, His)

目录号: HY-P72724
COA 产品使用指南

CD40蛋白是TNFSF5/CD40LG受体，通过TRAF6和MAP3K8途径转导信号，激活巨噬细胞和B细胞中的ERK，并诱导免疫球蛋白分泌。CD40 以单体和同二聚体形式存在，与 TRAF 蛋白（TRAF1、TRAF2、TRAF3、TRAF5 和 TRAF6）相互作用，并关键介导细胞对外部信号的反应。CD40 蛋白, Mouse (HEK293, His) 是重组的 CD40 蛋白，由 HEK293 表达，带有 C-6*His 标签。

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SPR 检测服务   重组蛋白定制服务

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3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 μg ￥1070 In-stock
50 μg ￥3000 In-stock
100 μg   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • 生物活性

  • 技术参数

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  • 产品资料

描述

CD40 protein is a TNFSF5/CD40LG receptor that transduces signals through the TRAF6 and MAP3K8 pathways, activates ERK in macrophages and B cells, and induces immunoglobulin secretion. CD40 exists in monomeric and homodimeric forms, interacts with TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6) and crucially mediates cellular responses to external signals. CD40 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD40 protein, expressed by HEK293 , with C-6*His labeled tag.
研究背景

CD40 蛋白作为 TNFSF5/CD40LG 的受体，通过 TRAF6 和 MAP3K8 途径转导信号，激活巨噬细胞和 B 细胞中的 ERK，从而诱导免疫球蛋白分泌。CD40 蛋白以单体和同二聚体形式存在，与 TRAF1、TRAF2、TRAF3、TRAF5 和 TRAF6 相互作用，在介导细胞对外部信号的反应中发挥着至关重要的作用。与 TRAF6 和 MAP3K8 的相互作用对于 ERK 的激活和随后的细胞过程尤其重要。
生物活性

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD40/TNFRSF5 is immobilized at 1 µg/mL (100 µL/well), can bind Recombinant Mouse CD40 Ligand/TNFSF5. The ED50 for this effect is 309.5 ng/mL.
种属

Mouse
表达系统

HEK293
标签

C-6*His
蛋白编号

P27512-1 (L20-R193)
基因 ID
蛋白结构
N-term
CD40 (L20-R193)
Accession # P27512-1
6*His
C-term
同用名
Tumor necrosis factor receptor superfamily member 5; Bp50; CD40; TNFRSF5
氨基酸序列

LGQCVTCSDKQYLHDGQCCDLCQPGSRLTSHCTALEKTQCHPCDSGEFSAQWNREIRCHQHRHCEPNQGLRVKKEGTAESDTVCTCKEGQHCTSKDCEACAQHTPCIPGFGVMEMATETTDTVCHPCPVGFFSNQSSLFEKCYPWTSCEDKNLEVLQKGTSQTNVICGLKSRMR

分子量

21-25 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.
组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
内毒素含量

<1 EU/μg, determined by LAL method.
复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
运输条件

Room temperature in continental US; may vary elsewhere.
文件资料

COA (209 KB)

产品使用指南 (1213 KB)

CD40 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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