1. 重组蛋白
  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens
  3. TNF Superfamily Stimulatory Immune Checkpoint Molecules T Cell CD Proteins B Cell CD Proteins Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. TNF Receptor Superfamily CD40 GITR/CD357
  5. CD40 蛋白, Rhesus Macaque (HEK293, His)

CD40 蛋白, Rhesus Macaque (HEK293, His)

目录号: HY-P75408
COA 产品使用指南

CD40 蛋白是重要的 TNFR 超家族成员,缺乏对特征注释传播至关重要的保守残基,表明独特的结构属性。这种独特性可能会影响 CD40 在 TNFR 超家族中的功能相互作用,强调需要进一步探索以阐明其在细胞信号通路中的特定作用和调节机制。CD40 蛋白, Rhesus Macaque (HEK293, His) 是重组的 CD40 蛋白,由 HEK293 表达,带有 C-10*His 标签。CD40 蛋白, Rhesus Macaque (HEK293, His) 全长 173 个氨基酸,分子量约为 ~31.67 kDa。

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SPR 检测服务   重组蛋白定制服务

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规格 价格 是否有货 数量
10 μg ¥600 In-stock
50 μg ¥1600 In-stock
100 μg ¥2700 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CD40 Protein, a vital TNFR superfamily member, lacks conserved residue(s) crucial for feature annotation propagation, indicating unique structural attributes. This distinctiveness may influence CD40's functional interactions within the TNFR superfamily, underscoring the need for further exploration to unravel its specific roles and regulatory mechanisms in cellular signaling pathways. CD40 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived CD40 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of CD40 Protein, Rhesus Macaque (HEK293, His) is 173 a.a., with molecular weight of ~31.67 kDa.

研究背景

CD40 是 TNFR 超家族的重要成员,其特点是不存在特征注释传播所需的保守残基。这一独特的特征表明 CD40 具有独特的结构属性,可能影响其在 TNFR 超家族中的功能相互作用。这些保守残基的缺乏强调了 CD40 的特殊性质,并强调了进一步探索以揭示其在细胞信号传导途径中的独特作用和调节机制的重要性。

生物活性

Immobilized Human CD40 Ligand at 2 μg/mL (100 μL/well) can bind Biotinylated Rhesus macaque CD40. The ED50 for this effect is 6.431 ng/mL, corresponding to a specific activity is 1.55×10^5 Unit/mg.

  • Immobilized Human CD40 Ligand at 2 μg/mL (100 μL/well) can bind Biotinylated Rhesus macaque CD40. The ED50 for this effect is 6.431 ng/mL, corresponding to a specific activity is 1.55×105 Unit/mg.
种属

Rhesus Macaque

表达系统

HEK293

标签

C-10*His

蛋白编号

NP_001252791.1 (E21-R193)

基因 ID
蛋白结构
N-term
CD40 (E21-R193)
Accession # NP_001252791.1
10*His
C-term
同用名
Tumor Necrosis Factor Receptor Superfamily member 5; Bp50; CD40L Receptor; CDw40; TNFRSF5
氨基酸序列

EPPTACREKQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCSESEFLDTWNRETRCHQHKYCDPNLGLRVQQKGTSETDTICTCEEGLHCMSESCESCVPHRSCLPGFGVKQIATGVSDTICEPCPVGFFSNVSSAFEKCRPWTSCETKDLVVQQAGTNKTDVVCGPQDRQR

分子量

Approximately 31.67 kDa

纯度
  • Greater than 90% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD40 Protein, Rhesus Macaque (HEK293, His)
目录号:
HY-P75408
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