CD47 蛋白, Human (Biotinylated, HEK293, Avi-His)

CD47 is an adhesion protein that regulates integrin signaling through G protein activation and serves as a receptor for thrombospondin THBS1, affecting signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, self-renewal and Immunomodulatory. It regulates pulmonary endothelin EDN1 signaling, acts as a pressor in blood pressure regulation, and is critical for memory formation. CD47 Protein, Human (Biotinylated, HEK293, Avi-His) is the recombinant human-derived CD47 protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag. The total length of CD47 Protein, Human (Biotinylated, HEK293, Avi-His) is 121 a.a., with molecular weight of 30-55 kDa.

CD47 是一种粘附蛋白，可促进细胞间相互作用，并作为血小板反应蛋白 THBS1 的受体，通过激活异三聚体 G 蛋白来调节整合素信号传导。CD47 参与多种细胞过程，有助于信号转导、心血管稳态、炎症、细胞凋亡、血管生成、细胞自我更新和免疫调节。值得注意的是，它在调节肺内皮素 EDN1 信号传导中发挥作用，并在响应 THBS1 的血压调节中充当升压剂。CD47 对于海马体的记忆形成和突触可塑性至关重要，作为 SIRPA 和 SIRPG 的受体，影响树突状细胞的成熟、细胞因子的产生、细胞间粘附和 T 细胞激活。此外，CD47 正向调节 T 细胞中 FAS 依赖性细胞凋亡并抑制血管生成，从而导致衰老过程中的代谢失调。作为对 THBS1 的反应，CD47 负向调节伤口愈合，抑制干细胞自我更新，并可能在膜运输和/或整合素依赖性信号转导中发挥作用。作为单体，CD47 与 THBS1、SIRPA、FAS/CD95、SIRPG、UBQLN1、UBQLN2 和潜在的纤维蛋白原相互作用，突出了其在细胞和分子途径中的复杂参与。

1.Immobilized Biotinylated Human CD47-His at 2 μg/mL (100 μl/well). Dose response curve for Human SIRP alpha-hFc with the EC₅₀ of 0.03 ng/mL determined by ELISA.
2.Immobilized Human SIRP alpha, hFc Tag at 1 μg/mL (100 μl/well) on the plate. Dose responsecurve for Biotinylated Human CD47, His Tag with the EC₅₀ of< 0.48 μg/mL determined by ELISA

Human

HEK293

C-Avi;C-6*His

Q08722-1 (Q19-P139)

961  [NCBI]

QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSP

30-55 kDa

Greater than 95% as determined by reducing SDS-PAGE or Tis-Bis PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-Citrate, 150 mM NaCl, pH 8.0 or PBS (pH 7.4), normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.