CD63 蛋白, Human/Cynomolgus (HEK293, N-His)

CD63 protein acts as a receptor for TIMP1, activating cell signaling pathways and promoting cell survival. It plays a key role in integrin signaling, leading to activation of AKT, FAK/PTK2, and MAP kinases. CD63 Protein, Human/Cynomolgus (HEK293, N-His) is the recombinant human-derived CD63 protein, expressed by HEK293 , with N-6*His labeled tag.

CD63 蛋白作为 TIMP1 的细胞表面受体发挥作用，有助于激活细胞信号级联。它在 ITGB1 的激活和随后的整联蛋白信号转导中发挥关键作用，从而导致 AKT、FAK/PTK2 和 MAP 激酶的激活。这种多面蛋白参与多种细胞过程，包括促进细胞存活、协调肌动蛋白细胞骨架的重组、通过参与 AKT 和 FAK/PTK2 激活来增强细胞粘附、扩散和迁移。此外，CD63 通过调节 KDR/VEGFR2 的内化参与 VEGFA 信号传导，并影响细胞内囊泡转运过程。它在 PMEL 管腔结构域的运输中发挥着不可或缺的作用，对于黑素细胞的发育和成熟至关重要。此外，CD63 通过调节 SELP 运输有助于白细胞粘附到内皮细胞上。虽然它可能在响应 Ms4a2/FceRI 刺激的肥大细胞脱颗粒中发挥作用，但它对于响应其他刺激的脱颗粒似乎是可有可无的。CD63 与 TIMP1 和 ITGB1 相互作用，将 TIMP1 募集到 ITGB1 复合物中，并与 CD9 和 ITGB3 形成复合物。它还与 PMEL 和 KDR/VEGFR2 相互作用，后者对于将 KDR 招募到 ITGB1 复合物中至关重要，进一步强调了其在细胞过程中的复杂作用。

Measured by its binding ability in a functional ELISA. When Recombinant Human BST-2/Tetherin is immobilized at 2.5 μg/mL (100 μL/well). The ED₅₀ for this effect is 2.134 μg/mL corresponding to a specific activity is 4.69×10^3 Unit/mg.

Human; Cynomolgus

HEK293

N-6*His

P08962-1/NP_001253224 (A103-V203)

967  [NCBI]/709828

AGYVFRDKVMSEFNNNFRQQMENYPKNNHTASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNV

20-30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.