CDC37 蛋白, Human (sf9, GST)

The CDC37 protein is a co-chaperone that binds multiple kinases and promotes their interaction with the Hsp90 complex, resulting in stability and enhanced activity. CDC37 Protein, Human (sf9, GST) is the recombinant human-derived CDC37 protein, expressed by Sf9 insect cells , with C-GST labeled tag. The total length of CDC37 Protein, Human (sf9, GST) is 378 a.a., with molecular weight of ~70.7 kDa.

CDC37 蛋白作为共伴侣，在促进众多激酶和 Hsp90 复合物之间的相互作用方面发挥着关键作用，从而导致这些激酶的稳定和活性增强。它通过抑制 HSP90AA1 ATP 酶活性发挥作用。CDC37 可能是多种蛋白质复合物的一部分，包括含有伴侣 HSP90 和 HSP70、共伴侣 STIP1/HOP、PPP5C、PTGES3/p23 以及客户蛋白（如 TSC2、CDK4、AKT、RAF1 和 NR3C1）的复合物。涉及 CDC37 的另一个复合物包含伴侣 HSP90 和 HSP70、辅助伴侣 PPP5C 和 TSC1，以及客户蛋白 TSC2、CDK4、AKT、RAF1 和 NR3C1，但明显缺乏 STIP1/HOP 和 PTGES3/p23。CDC37 还与 Hsp90/HSP90AB1 和 CDK6 形成独特的复合物。其相互作用延伸至多种蛋白质，包括 HSP90AA1、AR、CDK4、CDK6、EIF2AK1、RB1、KSR1 以及 FLCN、FNIP1 和 FNIP2。这种多样化的相互作用网络强调了 CDC37 在调节多种激酶和客户蛋白的稳定性和活性方面的多功能性。

Human

Sf9 insect cells

C-GST

Q16543 (M1-V378)

11140  [NCBI]

Approximately 70.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM GSH, 0.5 mM PMSF, 10% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.