CDC42 蛋白, Human (GST)

CDC42 is a plasma membrane-associated small GTPase that regulates cellular responses by cycling between an active GTP-bound state and an inactive GDP-bound state. It participates in epithelial cell polarization, regulates the attachment of spindle microtubules to kinetochores, affects cell migration, and plays a key role in the formation of filopodia. CDC42 Protein, Human (GST) is the recombinant human-derived CDC42 protein, expressed by E. coli , with N-GST labeled tag. The total length of CDC42 Protein, Human (GST) is 188 a.a., with molecular weight of ~44 kDa.

CDC42 是一种质膜相关的小 GTP 酶，可在活性 GTP 结合状态和非活性 GDP 结合状态之间动态转换，通过与各种效应蛋白相互作用来调节不同的细胞反应。值得注意的是，CDC42 错综复杂地参与上皮细胞极化等过程，在中期染色体会聚之前，它在调节纺锤体微管与动粒的附着方面发挥着关键作用。此外，CDC42 影响细胞迁移，对于神经元中丝状伪足（细长的富含肌动蛋白的突起）的延伸和维持至关重要。在神经元可塑性的背景下，它参与 CaMKII 介导的信号传导，影响树突棘结构可塑性并促进长期突触变化。此外，CDC42 通过与 DOCK10 和 DOCK11 相互作用，对于特定神经元细胞类型（例如浦肯野细胞和海马神经元）中棘的形成至关重要。在足细胞中，它在 DOCK11 激活后促进丝状伪足和足小体的形成。此外，CDC42 在吞噬过程中 F-肌动蛋白细胞骨架动力学的协调中发挥着至关重要的作用，有助于吞噬杯的形成。这种多方面的参与强调了 CDC42 在调节各种细胞功能方面的多功能性。

Human

E. coli

N-GST

P60953-2 (M1-C188)

998  [NCBI]

MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRC

Approximately 44 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 0.15M NaCl, 0.5 mM GSH, pH 8.0 or 20 mM Tris-HCL, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.