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  3. CDC42 蛋白, Human (GST)

CDC42 是一种质膜相关的小 GTP 酶,通过在活性 GTP 结合状态和非活性 GDP 结合状态之间循环来调节细胞反应。它参与上皮细胞极化,调节纺锤体微管与动粒的附着,影响细胞迁移,并在丝状伪足的形成中发挥关键作用。CDC42 蛋白, Human (GST) 是重组的 CDC42 蛋白,由 E. coli 表达,带有 N-GST 标签。CDC42 蛋白, Human (GST) 全长 188 个氨基酸,分子量约为 ~44 kDa。

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描述

CDC42 is a plasma membrane-associated small GTPase that regulates cellular responses by cycling between an active GTP-bound state and an inactive GDP-bound state. It participates in epithelial cell polarization, regulates the attachment of spindle microtubules to kinetochores, affects cell migration, and plays a key role in the formation of filopodia. CDC42 Protein, Human (GST) is the recombinant human-derived CDC42 protein, expressed by E. coli , with N-GST labeled tag. The total length of CDC42 Protein, Human (GST) is 188 a.a., with molecular weight of ~44 kDa.

研究背景

CDC42 是一种质膜相关的小 GTP 酶,可在活性 GTP 结合状态和非活性 GDP 结合状态之间动态转换,通过与各种效应蛋白相互作用来调节不同的细胞反应。值得注意的是,CDC42 错综复杂地参与上皮细胞极化等过程,在中期染色体会聚之前,它在调节纺锤体微管与动粒的附着方面发挥着关键作用。此外,CDC42 影响细胞迁移,对于神经元中丝状伪足(细长的富含肌动蛋白的突起)的延伸和维持至关重要。在神经元可塑性的背景下,它参与 CaMKII 介导的信号传导,影响树突棘结构可塑性并促进长期突触变化。此外,CDC42 通过与 DOCK10 和 DOCK11 相互作用,对于特定神经元细胞类型(例如浦肯野细胞和海马神经元)中棘的形成至关重要。在足细胞中,它在 DOCK11 激活后促进丝状伪足和足小体的形成。此外,CDC42 在吞噬过程中 F-肌动蛋白细胞骨架动力学的协调中发挥着至关重要的作用,有助于吞噬杯的形成。这种多方面的参与强调了 CDC42 在调节各种细胞功能方面的多功能性。

种属

Human

表达系统

E. coli

标签

N-GST

蛋白编号

P60953-2 (M1-C188)

基因 ID

998  [NCBI]

蛋白结构
N-term
GST
CDC42 (M1-C188)
Accession # P60953-2
C-term
中文名
重组人CDC42蛋白
同用名
Cell division control protein 42 homolog; G25K GTP-binding protein; CDC42
氨基酸序列

MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRC

分子量

Approximately 44 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 0.15M NaCl, 0.5 mM GSH, pH 8.0 or 20 mM Tris-HCL, 150 mM NaCl, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

CDC42 Protein, Human (GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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