CHEK2 蛋白, Human (sf9, GST)

The CHEK2 protein is a serine/threonine protein kinase that critically coordinates checkpoint-mediated cell cycle arrest, DNA repair activation, and apoptosis in response to DNA double-strand breaks. Activated CHEK2 phosphorylates CDC25A, CDC25B, and CDC25C, inhibiting their phosphatase activities and preventing the CDK-cyclin complex from activating cell cycle progression. CHEK2 Protein, Human (sf9, GST) is the recombinant human-derived CHEK2 protein, expressed by Sf9 insect cells , with GST labeled tag.

CHEK2 蛋白是一种丝氨酸/苏氨酸蛋白激酶，在检查点介导的细胞周期停滞、DNA 修复激活和响应 DNA 双链断裂的细胞凋亡中发挥着至关重要的作用。它还在正常细胞周期期间负调节细胞周期进程中发挥作用。激活后，CHEK2 会在特定共有序列处磷酸化各种效应子，从而抑制 CDC25A、CDC25B 和 CDC25C 的活性。这种对 CDC25 磷酸酶活性的抑制会导致 CDK-细胞周期蛋白复合物的抑制性酪氨酸磷酸化增加，从而阻止细胞周期进展。CHEK2 还磷酸化参与 G2/M 细胞周期停滞的 NEK6 和 BRCA2，增强 RAD51 与染色质的结合，通过同源重组实现 DNA 修复。此外，CHEK2 通过磷酸化和激活转录因子 FOXM1 来刺激 DNA 修复基因（包括 BRCA2）的转录。在细胞凋亡方面，CHEK2磷酸化p53/TP53、MDM4和PML，导致活性p53/TP53的积累并减少p53/TP53的降解。它还磷酸化转录因子 E2F1 以控制促凋亡基因的转录。此外，CHEK2 通过磷酸化 BRCA1 在有丝分裂纺锤体组装中发挥作用，而与 DNA 损伤无关。它的缺失可能导致某些癌细胞中观察到的染色体不稳定。CHEK2 还通过磷酸化单纯疱疹病毒 1/HHV-1 蛋白 ICP0 以激活其 SUMO 靶向泛素连接酶活性，在微生物感染中发挥作用。

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.The Km of CHEK2 is 201.7 μM.

Human

Sf9 insect cells

N-GST

O96017-1 (S2-L543)

11200  [NCBI]

87.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 5% glycerol, 5 mM DTT, 0.1 M Trehalose, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.