CHST15 蛋白, Human (HEK293, His)

The CHST15 protein is a sulfotransferase that catalyzes the transfer of sulfate to GalNAc 4-sulfate in chondroitin sulfate A to form chondroitin sulfate E with GlcA-GalNAc(4,6-SO(4)) units. It also sulfates the unique non-reducing terminal sequence GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), similar to thrombomodulin chondroitin sulfate. CHST15 Protein, Human (HEK293, His) is the recombinant human-derived CHST15 protein, expressed by HEK293 , with N-His labeled tag.

CHST15 蛋白作为磺基转移酶发挥独特作用，将硫酸盐从 3'-磷酸腺苷 5'-磷酸硫酸盐 (PAPS) 转移到硫酸软骨素 A 中 GalNAc 4-硫酸盐残基的 C-6 羟基，从而形成软骨素含有 GlcA-GalNAc(4,6-SO(4)) 重复单元的硫酸盐 E。此外，CHST15 将硫酸盐转移到独特的非还原末端序列 GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4)，从而形成高度硫酸化的结构，让人想起血栓调节蛋白硫酸软骨素。除了在硫酸软骨素生物合成中的作用之外，CHST15 还可以作为 B 细胞受体发挥作用，参与 BCR 连接介导的早期激活，介导对 B 细胞发育至关重要的调节信号以及 B 细胞特异性 RAG 表达的潜在调节。然而，这些结果在体内的确切含义仍不清楚。CHST15 的多方面活性凸显了其在控制硫酸软骨素修饰和 B 细胞调节途径的复杂分子过程中的重要性，但需要进一步研究来全面阐明其体内功能。

Measured by its ability to transfer sulfate from PAPS to chondroitin sulfate. The specific activity is 529.117 pmol/min/μg that incubate at 37 ºC for 20 minutes.

Human

HEK293

N-10*His

Q7LFX5-1 (S99-T561)

51363  [NCBI]

SGAHQELLISSPFHYGGFPSNPSLMDSENPSDTKEHHHQSSVNNISYMKDYPSIKLIINSITTRIEFTTRQLPDLEDLKKQELHMFSVIPNKFLPNSKSPCWYEEFSGQNTTDPYLTNSYVLYSKRFRSTFDALRKAFWGHLAHAHGKHFRLRCLPHFYIIGQPKCGTTDLYDRLRLHPEVKFSAIKEPHWWTRKRFGIVRLRDGLRDRYPVEDYLDLFDLAAHQIHQGLQASSAKEQSKMNTIIIGEASASTMWDNNAWTFFYDNSTDGEPPFLTQDFIHAFQPNARLIVMLRDPVERLYSDYLYFASSNKSADDFHEKVTEALQLFENCMLDYSLRACVYNNTLNNAMPVRLQVGLYAVYLLDWLSVFDKQQFLILRLEDHASNVKYTMHKVFQFLNLGPLSEKQEALMTKSPASNARRPEDRNLGPMWPITQKILRDFYRPFNARLAQVLADEAFAWKTT

Approximately 70-90 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.