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  3. Claudin-4/CLDN4 Protein-VLP, Human (HEK293, His)

Claudin-4/CLDN4 Protein-VLP, Human (HEK293, His)

目录号: HY-P700405
COA 产品使用指南

Claudin-4/CLDN4 Protein-VLP, Human (HEK293, His)推荐用于免疫动物、ELISA. 不推荐应用于受配体互作实验和SPR/BLI分析, 因为VLP包膜上还有宿主其他无关膜蛋白, 受配体互作会有很强的背景干扰. SPR/BLI分析则对芯片和实验方案有较高要求,成功率较低. 如果需要VLP对照,推荐HY-P701236。标签只能在变性条件下检测到。

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1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
20 μg ¥3250 In-stock
50 μg ¥6200 In-stock
100 μg ¥9900 询价
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

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  • 产品资料

描述

Claudin-4/CLDN4 Protein-VLP, Human (HEK293, His) is recommended for animal immunization, ELISA. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P701236. Tags can only be detected under denaturing conditions.

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Human CLDN4 at 5 μg/mL can bind Anti-CLDN4 recombinant antibody, the EC50 is ≤18 ng/mL.

种属

Human

表达系统

HEK293

标签

C-10*His

蛋白编号

O14493 (M1-V209)

基因 ID
蛋白结构
N-term
CLDN4 (M1-V209)
Accession # O14493
10*His
C-term
同用名
CLDN4; claudin 4; CPETR, CPETR1; claudin-4; Clostridium perfringens enterotoxin receptor 1; CPE R; hCPE R; WBSCR8; Williams Beuren syndrome chromosomal region 8 protein;
氨基酸序列

MASMGLQVMGIALAVLGWLAVMLCCALPMWRVTAFIGSNIVTSQTIWEGLWMNCVVQSTGQMQCKVYDSLLALPQDLQAARALVIISIIVAALGVLLSVVGGKCTNCLEDESAKAKTMIVAGVVFLLAGLMVIVPVSWTAHNIIQDFYNPLVASGQKREMGASLYVGWAASGLLLLGGGLLCCNCPPRTDKPYSAKYSAARSAAASNYV

分子量

23.4 kDa

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Claudin-4/CLDN4 Protein-VLP, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Claudin-4/CLDN4 Protein-VLP, Human (HEK293, His)
目录号:
HY-P700405
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