Claudin-3/CLDN3 蛋白, Human (His-B2M)

Claudin-3/CLDN3 proteins play a critical role in tight junctions, eliminating intercellular spaces through calcium-independent cell adhesion activity. It exhibits multifunctionality by forming homo- and heteropolymers with other CLDN members, including CLDN1 and CLDN2. Claudin-3/CLDN3 Protein, Human (His-B2M) is the recombinant human-derived Claudin-3/CLDN3 protein, expressed by E. coli , with N-6*His, B2M labeled tag. The total length of Claudin-3/CLDN3 Protein, Human (His-B2M) is 51 a.a., with molecular weight of ~19.7 kDa.

Claudin-3/CLDN3 蛋白利用不依赖于钙的细胞粘附活性，在紧密连接内细胞间隙的特异性消除中发挥着至关重要的作用。该蛋白质通过与其他 CLDN 成员形成同聚物和异聚物，包括与 CLDN1 和 CLDN2 同聚物的相互作用，展示了其多功能性。此外，Claudin-3/CLDN3 直接与紧密连接相关蛋白 TJP1/ZO-1、TJP2/ZO-2 和 TJP3/ZO-3 结合，强调其在紧密连接复合物的组装和维护中的不可或缺的作用。形成聚合物并与关键连接成分相互作用的能力凸显了 Claudin-3/CLDN3 在调节细胞粘附和细胞间隙结构完整性方面的重要性。

Human

E. coli

N-6*His;N-B2M

O15551 (R30-R80)

1365  [NCBI]

RVSAFIGSNIITSQNIWEGLWMNCVVQSTGQMQCKVYDSLLALPQDLQAAR

Approximately 21 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.