CNTF 蛋白, Human

CNTF is a pluripotent neurotrophic factor, belonging to the IL-6 cytokine family^[1]. CNTF could protect retinal cone and rod photoreceptors^[2]. CNTF has neuroprotective effects on a variety of central and also peripheral nervous system neurons^[3]. CNTF Protein, Human is produced by E. coli (A2-M200) with tag free.

睫状神经营养因子 (CNTF) 属于 IL-6 细胞因子家族。IL-6、IL-11 和 CNTF 与细胞因子反式信号传导相关。CNTF 与 IL-6 受体亚基 α (IL-6Rα) 表现出低亲和力相互作用，导致 IL-6Rβ/gp130/LIFR 信号受体复合物的形成和激活^[1]。CNTF 也是神经视网膜和感光细胞间基质中的一种细胞外信号蛋白，与 RPE、Muller 和感光细胞的膜相关^[2]。CNTF 对多种中枢和周围神经系统神经元具有神经保护作用。因为它在体外条件下促进少突胶质细胞前体细胞向少突胶质细胞的分化和成熟，从而改善髓鞘再生。重要的是，它还能提高成熟少突胶质细胞的存活率^[3]。人CNTF蛋白序列与小鼠和大鼠的相似性分别为81.82%和84.0%。

CNTF (重组人源蛋白; 20 ng/mL; 3 d) 显著抑制中脑培养物中胆碱乙酰转移酶 (ChAT) 的活性^[4]。

The ED₅₀ is <200 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >5 × 10³ units/mg.

• 
  Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.1749 μg/mL, corresponding to a specific activity is 5.717×10³ units/mg.

Human

E. coli

Tag Free

P26441 (A2-M200)

1270  [NCBI]

AFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVASTDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPTEGDFHQAIHTLLLQVAAFAYQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFISSHQTGIPARGSHYIANNKKM

Approximately 22-25 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS or 20 mM Tris-HCl, 100 mM NaCl, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0 or PBS, pH 7.4, 8% trehalose.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Jones SA, et al. Recent insights into targeting the IL-6 cytokine family in inflammatory diseases and cancer. Nat Rev Immunol. 2018 Dec;18(12):773-789.  [Content Brief]

  [2]. Abbaszadeh HA, et al. Human ciliary neurotrophic factor-overexpressing stable bone marrow stromal cells in the treatment of a rat model of traumatic spinal cord injury. Cytotherapy. 2015 Jul;17(7):912-21.  [Content Brief]

  [3]. Li S, et al. Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments. J Biol Chem. 2018 Sep 28;293(39):15256-15268.  [Content Brief]

  [4]. Pasquin S, et al. Ciliary neurotrophic factor (CNTF): New facets of an old molecule for treating neurodegenerative and metabolic syndrome pathologies. Cytokine Growth Factor Rev. 2015 Oct;26(5):507-15.  [Content Brief]

  [5]. Zurn A D, et al. Combined effects of GDNF, BDNF, and CNTF on motoneuron differentiation in vitro[J]. Journal of neuroscience research, 1996, 44(2): 133-141.