Complement C3/C3a 蛋白, Mouse

Complement C3/C3a protein activates the complement system, playing a central role in both classical and alternative pathways. C3b binds covalently to cell surface carbohydrates or immune aggregates, while C3a acts as an inflammatory mediator, inducing neutrophil chemoattraction and promoting smooth muscle contraction, increased vascular permeability, and histamine release. The shorter isoform of C3a stimulates B-cells. Complement C3/C3a Protein, Mouse is the recombinant mouse-derived Complement C3/C3a protein, expressed by E. coli , with tag free.

补体 C3/C3a 蛋白在补体系统的激活中发挥着关键作用，是经典途径和替代途径中的中心反应。激活后，C3b 可以通过其反应性硫酯与细胞表面碳水化合物或免疫聚集体共价结合。C3a 源自补体 C3 的蛋白水解降解，充当局部炎症介质，诱导中性粒细胞趋化并促进平滑肌收缩、增加血管通透性以及肥大细胞和嗜碱性白细胞释放组胺。此外，较短的 C3a 异构体具有 B 细胞刺激活性。

Measured by its ability to enhance IL-6 secretion by THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is ≤1.353 μg/mL.

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  Measured by its ability to enhance IL-6 secretion by THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 0.5190 μg/mL, corresponding to a specific activity is 1926.7823 U/mg.

Mouse

E. coli

Tag Free

P01027-1 (S671-R748)

12266  [NCBI]

SVQLMERRMDKAGQYTDKGLRKCCEDGMRDIPMRYSCQRRARLITQGENCIKAFIDCCNHITKLREQHRRDHVLGLAR

Approximately 9-14 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.