CRACC/SLAMF7 蛋白, Human (HEK293, His)

The CRACC/SLAMF7 protein is an autoligand receptor in the SLAM family that complexly regulates immune cell activation and differentiation through finely regulated interactions. Isoform 1 mediates NK cell activation through an SH2D1A-independent ERK-mediated pathway and positively regulates NK cell function dependent on phosphorylated SH2D1B. CRACC/SLAMF7 Protein, Human (HEK293, His) is the recombinant human-derived CRACC/SLAMF7 protein, expressed by HEK293 , with C-6*His labeled tag.

CRACC/SLAMF7 蛋白作为信号淋巴细胞激活分子 (SLAM) 家族中的自配体受体，参与同型或异型细胞间相互作用，调节各种免疫细胞的激活和分化，错综复杂地促进调节和分化。先天性免疫反应和适应性免疫反应之间的相互联系。该蛋白质的活性可通过小细胞质接头蛋白（包括 SH2D1A/SAP 和/或 SH2D1B/EAT-2）的存在或不存在进行微调。具体来说，CRACC/SLAMF7 的亚型 1 通过不依赖于 SH2D1A 的细胞外信号调节 ERK 介导途径介导 NK 细胞活化，以依赖于磷酸化 SH2D1B 的机制正向调节 NK 细胞功能。下行信令涉及PLCG1、PLCG2和PI3K。此外，NK 细胞之间的同型相互作用可能有助于激活，但在缺乏 SH2D1B 的情况下，CRACC/SLAMF7 会抑制 NK 细胞功能。该蛋白还充当 T 细胞的抑制因子，并可能在淋巴细胞粘附中发挥作用。在脂多糖激活的单核细胞中，它负向调节促炎细胞因子的产生。然而，CRACC/SLAMF7 的异构体 3 不介导任何 NK 细胞激活，表明免疫调节中异构体特异性的功能差异。

Human

HEK293

C-6*His

Q9NQ25-1 (S23-M226)

57823  [NCBI]

SGPVKELVGSVGGAVTFPLKSKVKQVDSIVWTFNTTPLVTIQPEGGTIIVTQNRNRERVDFPDGGYSLKLSKLKKNDSGIYYVGIYSSSLQQPSTQEYVLHVYEHLSKPKVTMGLQSNKNGTCVTNLTCCMEHGEEDVIYTWKALGQAANESHNGSILPISWRWGESDMTFICVARNPVSRNFSSPILARKLCEGAADDPDSSM

35-44 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 or PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.