CREB3L1 蛋白, Human (HEK293, His)

CREB3L1 protein is located on the endoplasmic reticulum membrane and is converted into a transcription factor processing cyclic AMP response element binding protein 3-like protein 1. During endoplasmic reticulum stress or DNA damage, it translocates to the Golgi apparatus, is cleaved by S1P/MBTPS1 and S2P/MBTPS2, and releases a cytoplasmic domain that activates gene transcription in the nucleus. CREB3L1 Protein, Human (HEK293, His) is the recombinant human-derived CREB3L1 protein, expressed by HEK293 , with C-His labeled tag.

CREB3L1 蛋白作为转录因子形式的前体，加工后的环 AMP 响应元件结合蛋白 3 样蛋白 1，嵌入内质网 (ER) 膜中，其 N 端 DNA 结合和转录激活结构域面向朝向膜的细胞质面。为了应对 ER 应激或 DNA 损伤，CREB3L1 被转运到高尔基体，在那里它被常驻蛋白酶 S1P/MBTPS1 和 S2P/MBTPS2 进行位点特异性切割。由此产生的 N 端胞质结构域易位至细胞核，激活参与细胞周期进展抑制的特定靶基因的转录。该转录因子与 DNA 共有序列 5'-GTGXGCXGC-3' 结合，在细胞类型特异性 DNA 损伤和未折叠蛋白反应 (UPR) 中发挥着至关重要的作用。在骨形成过程中，CREB3L1 调节 COL1A1 和可能的 COL1A2 转录，有助于骨基质蛋白的分泌。此外，它通过与 BiP/HSPA5 启动子的 cAMP 反应元件 (CRE) 结合，保护星形胶质细胞免受 ER 应激诱导的细胞死亡。在星形胶质细胞和成骨细胞中，CREB3L1 通过激活 p21/CDKN1A 等细胞周期抑制剂的转录，在 DNA 损伤后抑制 G2/M 期后的细胞周期进展。此外，TGFB1 需要激活参与胶原蛋白细胞外基质组装的基因。

Human

HEK293

C-His

Q96BA8-1 (E396-S519)

90993  [NCBI]

21-31 kDa.

Greater than 95% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of PBS, PH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.