Cyclophilin A 蛋白, Mouse

Cyclophilin A protein catalyzes the isomerization of proline imide peptide bonds in oligopeptides. Cyclophilin A Protein, Mouse is the recombinant mouse-derived Cyclophilin A protein, expressed by E. coli , with tag free.

亲环蛋白 A 催化脯氨酸酰亚胺肽键的顺反异构化，通过激活其膜受体 BSG/CD147 对白细胞发挥有效的趋化作用，并启动信号级联反应，最终导致 MAPK/ERK 激活。该蛋白通过刺激 NF-kappa-B 和 MAP 激酶信号传导，以促炎方式激活内皮细胞 (EC)，诱导 SELE 和 VCAM1 等粘附分子的表达。此外，Cyclophilin A 通过促进 CCL2 和 BCL2L11 的 FOXO1 依赖性表达来诱导 EC 细胞凋亡。为了应对氧化应激，它通过 NF-kappa-B 激活、AKT1 上调和 BCL2 诱导启动 EC 中的促凋亡和抗凋亡信号通路。它负向调节 MAP3K5/ASK1 激酶活性，对于 TARDBP 在异质核核糖核蛋白复合物中的组装至关重要，从而影响 TARDBP 与 RNA 的结合以及 HDAC6、ATG7 和 VCP 的表达，这对于蛋白质聚集物的清除至关重要。亲环蛋白 A 在血小板活化和聚集、调节钙动员、整合素 ITGA2B:ITGB3 双向信号传导以及结合硫酸乙酰肝素糖胺聚糖方面也发挥着关键作用。

Measured by its ability to inhibit calcineurin phosphatase activity in the presence of Cyclosporin A. The IC₅₀ for inhibition of calcineurin activity is 530.199 nM that incubate at 37 ºC for 60 minutes.

Mouse

E. coli

Tag Free

P17742 (M1-L164)

268373  [NCBI]

MVNPTVFFDITADDEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSSFHRIIPGFMCQGGDFTRHNGTGGRSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITISDCGQL

approximately 18.07 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.