1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins Endothelial cell CD Proteins Pattern Recognition Receptors
  4. DC-SIGN1/CD209 DC-SIGN1/CD209 C-type Lectin Receptors
  5. DC-SIGN/CD209 蛋白, Rhesus Macaque (HEK293, His)

DC-SIGN/CD209 蛋白, Rhesus Macaque (HEK293, His)

目录号: HY-P74205
COA 产品使用指南

DC-SIGN/CD209 蛋白是一种存在于巨噬细胞和树突状细胞 (DC) 表面的C型凝集素受体,是关键的病原体识别受体,在启动初级免疫反应中发挥着至关重要的作用。它介导溶酶体室内病原体的内吞作用和随后的降解。在 DC 上,它充当 ICAM2 和 ICAM3 的高亲和力受体,与甘露糖样碳水化合物结合。DC-SIGN/CD209 蛋白, Rhesus Macaque (HEK293, His) 是重组的 DC-SIGN/CD209 蛋白,由 HEK293 表达,带有 N-His 标签。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

DC-SIGN/CD209 Protein, is a C-type lectin receptor present on the surface of both macrophages and dendritic cells (DCs), serves as a pivotal pathogen-recognition receptor, playing a crucial role in initiating the primary immune response. It mediates endocytosis and subsequent degradation of pathogens within lysosomal compartments. On DCs, it acts as a high-affinity receptor for ICAM2 and ICAM3, binding to mannose-like carbohydrates. DC-SIGN/CD209 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived DC-SIGN/CD209 protein, expressed by HEK293 , with N-His labeled tag.

研究背景

DC-SIGN/CD209 蛋白是一种存在于巨噬细胞和树突状细胞 (DC) 表面的C型凝集素受体,是关键的病原体识别受体,在启动初级免疫反应中发挥着至关重要的作用。该受体参与病原体的内吞作用,导致其随后在溶酶体室内降解。在此过程之后,DC-SIGN 返回细胞膜表面,通过 MHC II 类蛋白将病原体衍生的抗原呈递给静息 T 细胞,从而触发适应性免疫反应。在 DC 上,它充当 ICAM2 和 ICAM3 的高亲和力受体,与甘露糖样碳水化合物结合。值得注意的是,DC-SIGN 可能充当 DC 滚动受体,通过与内皮 ICAM2 相互作用,促进 DC 前体从血流跨内皮迁移到组织。此外,它似乎通过与 DC 和 T 细胞之间形成的免疫突触内 T 细胞上的 ICAM3 结合来调节 DC 诱导的 T 细胞增殖[1][2]

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rhesus Macaque DC-SIGN at 2 μg/mL can bind gp120. The ED50 for this effect is 1.092 μg/mL.

  • Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rhesus Macaque DC-SIGN at 2 μg/mL canbind gp120. The ED50 for this effect is 1.092 μg/mL
种属

Rhesus Macaque

表达系统

HEK293

标签

N-6*His

蛋白编号

AAK74185.1 (K62-E381)

基因 ID
蛋白结构
N-term
His
CD209 (K62-E381)
Accession # AAK74185.1
C-term
同用名
CD209 antigen; DC-SIGN1; CD209
氨基酸序列

KVPSSLSQGQSKQDAIYQNLTQLKVAVSELSEKSKQQEIYQELTRLKAAVGELPEKSKQQEIYEELTRLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKQQEIYQELSRLKAAVGDLPEKSKQQEIYQKLTQLKAAVDGLPDRSKQQEIYQELIQLKAAVERLCRPCPWEWTFFQGNCYFMSNSQRNWHNSITACQEVGAQLVVIKSAEEQNFLQLQSSRSNRFTWMGLSDLNHEGTWQWVDGSPLLPSFKQYWNKGEPNNIGEEDCAEFSGNGWNDDKCNLAKFWICKKSAASCSGDEERLLSPAPTTPNPPPE

分子量

Approximately 44 kDa due to the glycosylation.

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

DC-SIGN/CD209 Protein, Rhesus Macaque (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
DC-SIGN/CD209 Protein, Rhesus Macaque (HEK293, His)
目录号:
HY-P74205
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