Dectin-1/CLEC7A 蛋白, Human (HEK293, Fc)

The Dectin-1/CLEC7A protein is a lectin and pattern recognition receptor that targets β-1,3- and β-1,6-linked glucans in bacterial and fungal cell walls. It triggers Toll-like receptor 2 (TLR2)-mediated inflammation by recruiting splenic tyrosine kinase (SYK) and activating the CARD domain-BCL10-MALT1 (CBM) signalosome. Dectin-1/CLEC7A Protein, Human (HEK293, Fc) is the recombinant human-derived Dectin-1/CLEC7A protein, expressed by HEK293 , with N-hFc labeled tag. The total length of Dectin-1/CLEC7A Protein, Human (HEK293, Fc) is 182 a.a., with molecular weight of 50-65 kDa.

Dectin-1/CLEC7A 蛋白作为凝集素发挥作用，充当模式识别受体 (PRR)，专为 β-1,3-连接和 β-1,6-连接葡聚糖（病原细菌和真菌细胞壁的主要成分）而设计。Dectin-1 对于 Toll 样受体 2 (TLR2) 介导的炎症反应至关重要，在与 β-葡聚糖结合后，通过其基于免疫受体酪氨酸的激活基序 (ITAM) 招募脾酪氨酸激酶 (SYK)，并引发信号级联反应。该级联激活 CARD 结构域-BCL10-MALT1 (CBM) 信号体，从而激活 NF-kappa-B 和 MAP 激酶 p38 通路。因此，编码促炎细胞因子和趋化因子的基因表达受到刺激，从而增强巨噬细胞和树突细胞中细胞因子的产生。除了在炎症中发挥作用外，Dectin-1 还介导活性氧的产生，并有助于白色念珠菌分生孢子的吞噬作用。此外，Dectin-1 结合 T 细胞，在 T 细胞激活中发挥作用，在 β-葡聚糖结合后诱导 SCIMP 磷酸化。Dectin-1 作为同二聚体存在，与 SYK 相互作用，参与真菌病原体存在下的白细胞激活。

Human

HEK293

N-hFc

Q9BXN2 (T66-M247)

64581  [NCBI]

TMAIWRSNSGSNTLENGYFLSRNKENHSQPTQSSLEDSVTPTKAVKTTGVLSSPCPPNWIIYEKSCYLFSMSLNSWDGSKRQCWQLGSNLLKIDSSNELGFIVKQVSSQPDNSFWIGLSRPQTEVPWLWEDGSTFSSNLFQIRTTATQENPSPNCVWIHVSVIYDQLCSVPSYSICEKKFSM

50-65 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.