DENR 蛋白, Human (His-SUMO)

DENR proteins play a key role in the translation process, participating in mRNA scanning and start codon recognition. It crucially promotes the recruitment of aminoacylated initiator tRNA to the 40S ribosomal P site during translation initiation. DENR Protein, Human (His-SUMO) is the recombinant human-derived DENR protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of DENR Protein, Human (His-SUMO) is 197 a.a., with molecular weight (affected by relative charge) of ~46 KDa.

DENR 蛋白成为翻译过程中的关键角色，可能通过扫描和识别起始密码子参与目标 mRNA 的翻译。它通过促进氨酰化起始子 tRNA 募集到 40S 核糖体的 P 位点，在翻译起始中发挥关键作用。此外，在 ABCE1 介导的终止后核糖体复合物解离成亚基后，DENR 还参与促进回收的 40S 亚基中脱酰化 tRNA 和 mRNA 的释放。值得注意的是，当癌基因 MCTS1 招募到翻译起始复合物时，DENR 有助于调节癌症相关 mRNA 子集的翻译谱，突显了其在癌症相关翻译调节中的潜在参与。它与 MCTS1 的相互作用进一步强调了它在与翻译起始和控制相关的复杂细胞过程中的重要性。

Human

E. coli

N-6*His;N-SUMO

O43583 (A2-K198)

8562  [NCBI]

AADISESSGADCKGDPRNSAKLDADYPLRVLYCGVCSLPTEYCEYMPDVAKCRQWLEKNFPNEFAKLTVENSPKQEAGISEGQGTAGEEEEKKKQKRGGRGQIKQKKKTVPQKVTIAKIPRAKKKYVTRVCGLATFEIDLKEAQRFFAQKFSCGASVTGEDEIIIQGDFTDDIIDVIQEKWPEVDDDSIEDLGEVKK

Approximately 46 kDa.The reducing (R) protein migrat es as 46 kDa in SDS-PAGE may be due to relative charge.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaC, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.