DEP-1/CD148 蛋白, Human (341a.a, His)

DEP-1/CD148 protein is a tyrosine phosphatase that affects multiple targets such as CTNND1, FLT3, PDGFRB, and EGFR, thereby affecting cellular processes such as adhesion, migration, proliferation, and differentiation. DEP-1/CD148 is critical in vascular development, regulating macrophage adhesion, platelet activation, and thrombosis, while negatively regulating cell proliferation and PDGF-stimulated migration. DEP-1/CD148 Protein, Human (341a.a, His) is the recombinant human-derived DEP-1/CD148 protein, expressed by E. coli , with N-His labeled tag.

DEP-1/CD148 蛋白是一种酪氨酸磷酸酶，对多种分子靶点产生影响，包括 CTNND1、FLT3、PDGFRB、MET、KDR、LYN、SRC、MAPK1、MAPK3、EGFR、TJP1、OCLN、PIK3R1 和PIK3R2。它的参与延伸到各种细胞过程，例如粘附、迁移、增殖和分化。在血管发育中，DEP-1/CD148 起着至关重要的作用，作为巨噬细胞粘附和扩散的调节剂，同时积极影响细胞-基质粘附。此外，它还作为血小板活化和血栓形成的正调节剂，同时通过 PDGFR 去磷酸化作为细胞增殖和 PDGF 刺激的细胞迁移的负调节剂。DEP-1/CD148 还通过去磷酸化 KDR 正向调节内皮细胞存活以及 VEGF 诱导的 SRC 和 AKT 激活。它负向调节 EGFR 信号通路并增强重组过程中上皮连接的屏障功能。在 T 细胞受体 (TCR) 信号传导中，DEP-1/CD148 充当负调节因子，在 TCR 激活时上调并排除在免疫突触之外，随后使 PLCG1 和 LAT 去磷酸化，从而下调 T 细胞信号传导延长。抗原呈递细胞（APC）脱离。此外，它还能激活血管生成和细胞迁移，同时下调内皮粘附分子 ICAM1 和 VCAM1 的表达。DEP-1/CD148 的多方面活性强调了其在控制多种细胞过程和信号通路中的关键作用。

Human

E. coli

N-His

Q12913-1 (R997-A1337)

5795  [NCBI]

Approximately 37 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.