2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. DHRS9 蛋白, Human (His)

DHRS9 蛋白, Human (His)

目录号: HY-P76871
产品使用指南

DHRS9 蛋白是一种 3-α-羟基类固醇脱氢酶，经研究支持，可将 3-α-四氢孕酮转化为二羟基孕酮，将 3-α-雄烷二醇转化为二羟基孕酮。此外，DHRS9 在反应中利用 NADH 和 NADPH 作为辅酶，对视黄醛生物合成视黄酸有显着贡献。DHRS9 蛋白, Human (His) 是重组的 DHRS9 蛋白，由 E. coli 表达，带有 N-His 标签。DHRS9 蛋白, Human (His) 全长 302 个氨基酸，分子量约为 ~34 kDa。

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描述

DHRS9 Protein, a 3-alpha-hydroxysteroid dehydrogenase, converts 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone, supported by studies. Additionally, DHRS9 significantly contributes to retinoic acid biosynthesis from retinaldehyde, utilizing both NADH and NADPH as coenzymes in its reactions. DHRS9 Protein, Human (His) is the recombinant human-derived DHRS9 protein, expressed by E. coli , with N-His labeled tag. The total length of DHRS9 Protein, Human (His) is 302 a.a., with molecular weight of ~34 kDa.
研究背景

DHRS9 蛋白是一种 3-α-羟基类固醇脱氢酶，可将 3-α-四氢孕酮（别孕酮）转化为二羟基孕酮，将 3-α-雄甾烷二醇转化为二羟基孕酮。这种酶活性已得到研究的支持。此外，研究证明，DHRS9 在视黄醛生物合成视黄酸中也发挥着重要作用。值得注意的是，该蛋白质能够在其酶促反应中利用 NADH 和 NADPH 作为辅酶。
种属

Human
表达系统

E. coli
标签

N-His
蛋白编号

Q9BPW9 (R18-V319)
基因 ID
蛋白结构
N-term
His
DHRS9 (R18-V319)
Accession # Q9BPW9
C-term
同用名
Dehydrogenase/reductase SDR family member 9; 3-alpha-HSD; RDH-E2; RDH15; SDR9C4
分子量

Approximately 34 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

DHRS9 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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