DMP-1 蛋白, Human (HEK293, His)

DMP-1 protein exhibits dual functions in osteoblast differentiation. In undifferentiated osteoblast nuclei, its nonphosphorylated form activates osteoblast-specific genes. DMP-1 Protein, Human (HEK293, His) is the recombinant human-derived DMP-1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of DMP-1 Protein, Human (HEK293, His) is 497 a.a., with molecular weight of 53-90 kDa.

DMP-1 蛋白似乎在成骨细胞分化过程中表现出双重功能。在未分化的成骨细胞的细胞核中，其非磷酸化形式充当转录成分，激活成骨细胞特异性基因，例如骨钙素。当成骨细胞转变为骨细胞时，DMP-1 发生磷酸化，随后被输出到细胞外基质中。在这种细胞外环境中，它在羟基磷灰石的成核中发挥调节作用，表明它参与矿化过程。DMP-1 的复杂功能强调了它在控制成骨细胞分化和骨形成的分子事件中的重要性。值得注意的是，DMP-1 与输入蛋白 α 相互作用，为其细胞动力学和潜在的调节机制增加了一层复杂性。

Measured by its binding ability in a functional ELISA. Immobilized Integrin alpha v beta 3 at 2 µg/mL can bind DMP-1. The ED₅₀ for this effect is 6.245-6.798 nM.

Human

HEK293

C-6*His

Q13316 (L17-Y513)

1758  [NCBI]

LPVTRYQNNESEDSEEWKGHLAQAPTPPLESSESSEGSKVSSEEQANEDPSDSTQSEEGLGSDDHQYIYRLAGGFSRSTGKGGDDKDDDEDDSGDDTFGDDDSGPGPKDRQEGGNSRLGSDEDSDDTIQASEESAPQGQDSAQDTTSESRELDNEDRVDSKPEGGDSTQESESEEHWVGGGSDGESSHGDGSELDDEGMQSDDPESIRSERGNSRMNSAGMKSKESGENSEQANTQDSGGSQLLEHPSRKIFRKSRISEEDDRSELDDNNTMEEVKSDSTENSNSRDTGLSQPRRDSKGDSQEDSKENLSQEESQNVDGPSSESSQEANLSSQENSSESQEEVVSESRGDNPDPTTSYVEDQEDSDSSEEDSSHTLSHSKSESREEQADSESSESLNFSEESPESPEDENSSSQEGLQSHSSSAESQSEESHSEEDDSDSQDSSRSKEDSNSTESKSSSEEDGQLKNIEIESRKLTVDAYHNKPIGDQDDNDCQDGY

35-95 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Histidine-HCl, 6% Trehalose, 4% Mannitol, 0.05% Tween 80, pH 6.0 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.