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  4. DPYSL2 蛋白, Human (P.pastoris, His)

DPYSL2 蛋白, Human (P.pastoris, His)

目录号: HY-P71816
产品使用指南

DPYSL2 蛋白在神经元发育、极性、轴突生长、引导、生长锥塌陷和细胞迁移中发挥着至关重要的作用。它对于 3 类信号蛋白和细胞骨架重塑的信号传导至关重要。DPYSL2 与 CRMP1、DPYSL3、DPYSL4 或 DPYSL5 形成同源四聚体和异源四聚体。它与 CYFIP1/SRA1、HTR4、CLN6 和 MICALL1 相互作用，突出了其在细胞过程中的多种功能和相互作用。DPYSL2 蛋白, Human (P.pastoris, His) 是重组的 DPYSL2 蛋白，由 P. pastoris 表达，带有 N-His 标签。DPYSL2 蛋白, Human (P.pastoris, His) 全长 572 个氨基酸，分子量约为 ~64.3 kDa。

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描述

DPYSL2 protein plays a crucial role in neuronal development, polarity, axon growth, guidance, growth cone collapse, and cell migration. It is essential for signaling by class 3 semaphorins and cytoskeleton remodeling. DPYSL2 forms homotetramers and heterotetramers with CRMP1, DPYSL3, DPYSL4, or DPYSL5. It interacts with CYFIP1/SRA1, HTR4, CLN6, and MICALL1, highlighting its diverse functions and interactions in cellular processes. DPYSL2 Protein, Human (P.pastoris, His) is the recombinant human-derived DPYSL2 protein, expressed by P. pastoris , with N-His labeled tag. The total length of DPYSL2 Protein, Human (P.pastoris, His) is 572 a.a., with molecular weight of ~64.3 kDa.
研究背景

DPYSL2 蛋白积极参与神经元发育、极性、轴突生长、引导、生长锥塌陷和细胞迁移。其关键作用延伸至 3 类信号蛋白的信号传导和随后的细胞骨架重塑。DPYSL2 与 CRMP1、DPYSL3、DPYSL4 或 DPYSL5 形成同源四聚体和异源四聚体。与 CYFIP1/SRA1 的相互作用通过其 C 末端发生，DPYSL2 还与 HTR4、CLN6 和 MICALL1 相互作用。这些蛋白质相互作用凸显了 DPYSL2 多方面参与对神经元功能和发育至关重要的各种细胞过程。
种属

Human
表达系统

P. pastoris
标签

N-His
蛋白编号

Q16555 (M1-G572)
基因 ID
蛋白结构
N-term
His
DPYSL2 (1M-572G)
Accession # Q16555
C-term
同用名
Collapsin response mediator protein 2; Collapsin response mediator protein hCRMP 2; CRAM; CRMP 2; CRMP-2; N2A3; TOAD 64; TOAD64; ULIP 2 protein; ULIP-2; Ulip2
氨基酸序列

MSYQGKKNIPRITSDRLLIKGGKIVNDDQSFYADIYMEDGLIKQIGENLIVPGGVKTIEAHSRMVIPGGIDVHTRFQMPDQGMTSADDFFQGTKAALAGGTTMIIDHVVPEPGTSLLAAFDQWREWADSKSCCDYSLHVDISEWHKGIQEEMEALVKDHGVNSFLVYMAFKDRFQLTDCQIYEVLSVIRDIGAIAQVHAENGDIIAEEQQRILDLGITGPEGHVLSRPEEVEAEAVNRAITIANQTNCPLYITKVMSKSSAEVIAQARKKGTVVYGEPITASLGTDGSHYWSKNWAKAAAFVTSPPLSPDPTTPDFLNSLLSCGDLQVTGSAHCTFNTAQKAVGKDNFTLIPEGTNGTEERMSVIWDKAVVTGKMDENQFVAVTSTNAAKVFNLYPRKGRIAVGSDADLVIWDPDSVKTISAKTHNSSLEYNIFEGMECRGSPLVVISQGKIVLEDGTLHVTEGSGRYIPRKPFPDFVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKTVTPASSAKTSPAKQQAPPVRNLHQSGFSLSGAQIDDNIPRRTTQRIVAPPGGRANITSLG

分子量

Approximately 64.3 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

DPYSL2 Protein, Human (P.pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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