DPYSL3 蛋白, Human (His)

The DPYSL3 protein plays a key role in class 3 semaphorin-triggered signaling, driving cytoskeletal remodeling that is critical for axon guidance, growth cone collapse, and cell migration. As a component of homo- and heterotetramers of CRMP1, DPYSL2, DPYSL4 or DPYSL5, it is intricately associated with related proteins, highlighting its involvement in complex cellular dynamics. DPYSL3 Protein, Human (His) is the recombinant human-derived DPYSL3 protein, expressed by E. coli , with N-His labeled tag. The total length of DPYSL3 Protein, Human (His) is 570 a.a., with molecular weight of ~66.0 kDa.

DPYSL3 蛋白对于 3 类信号蛋白启动的信号级联以及随后的细胞骨架重塑是不可或缺的。它积极促进轴突引导、神经元生长锥塌陷和细胞迁移，强调了其在细胞过程中的多方面作用。作为 CRMP1、DPYSL2、DPYSL4 或 DPYSL5 的同源四聚体和异源四聚体的一部分，它与相关蛋白形成复杂的关联，反映了它参与复杂的细胞动力学。此外，DPYSL3 与突触小泡蛋白 2 和 intersectin 的 SH3A 结构域相互作用，进一步强调了它参与对细胞功能至关重要的分子相互作用。值得注意的是，DPYSL3 与 FLNA 结合，正如其与 FLNA 的钙调蛋白同源 (CH) 结构域 1 和细丝蛋白重复序列 24 的相互作用所证明的那样，强调了其在 FLNA 介导的细胞骨架重排中的作用。

Human

E. coli

N-His

Q14195 (1M-570S)

1809  [NCBI]

MSYQGKKNIPRITSDRLLIKGGRIVNDDQSFYADIYMEDGLIKQIGDNLIVPGGVKTIEANGKMVIPGGIDVHTHFQMPYKGMTTVDDFFQGTKAALAGGTTMIIDHVVPEPESSLTEAYEKWREWADGKSCCDYALHVDITHWNDSVKQEVQNLIKDKGVNSFMVYMAYKDLYQVSNTELYEIFTCLGELGAIAQVHAENGDIIAQEQTRMLEMGITGPEGHVLSRPEELEAEAVFRAITIASQTNCPLYVTKVMSKSAADLISQARKKGNVVFGEPITASLGIDGTHYWSKNWAKAAAFVTSPPLSPDPTTPDYINSLLASGDLQLSGSAHCTFSTAQKAIGKDNFTAIPEGTNGVEERMSVIWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRISVGSDSDLVIWDPDAVKIVSAKNHQSAAEYNIFEGMELRGAPLVVICQGKIMLEDGNLHVTQGAGRFIPCSPFSDYVYKRIKARRKMADLHAVPRGMYDGPVFDLTTTPKGGTPAGSARGSPTRPNPPVRNLHQSGFSLSGTQVDEGVRSASKRIVAPPGGRSNITSLS

Approximately 66.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against solution in 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.