ELAVL4 蛋白, Human (His-SUMO)

ELAVL4 protein is a broad RNA-binding factor that post-transcriptionally regulates mRNAs such as GAP43, VEGF, FOS, CDKN1A, and ACHE, stabilizing and protecting them from decay by binding to ARE sequences. It reduces mRNA deadenylation and enhances mRNA binding affinity by interacting with the Poly(A) tail. ELAVL4 Protein, Human (His-SUMO) is the recombinant human-derived ELAVL4 protein, expressed by E. coli , with N-10*His, N-SUMO, C-Myc labeled tag.

ELAVL4 蛋白是一种 RNA 结合因子，广泛参与 mRNA 的转录后调节，影响 mRNA 的稳定性、选择性剪接和翻译。通过与目标 mRNA（包括 GAP43、VEGF、FOS、CDKN1A 和 ACHE）3' 非翻译区 (UTR) 中富含 AU 的元件 (ARE) 序列结合，ELAVL4 有助于稳定和保护这些转录本免遭衰变。它通过与 mRNA 3'UTR 结合来减少 mRNA 去腺苷化，并且还与 3'UTR 中的聚腺苷酸 (poly(A)) 尾部相互作用，从而增强 mRNA 结合亲和力。ELAVL4 主要在神经元特异性 RNA 加工中发挥作用，稳定与神经元蛋白相关的 mRNA，并有助于神经祖细胞分化、神经系统发育以及学习和记忆机制。此外，它还在神经元干细胞增殖活性的负调节中发挥作用，促进神经元分化。ELAVL4 的影响延伸到神经突生长、树突乔木的建立和成熟，影响新皮质和海马回路功能。它形成 TAU mRNP 复合物的一部分，与 EIF4F 帽结合复合物结合，并与各种蛋白质相互作用，包括 SMN、MAP1 轻链 LC1 和 LC2，突出了其在 RNA 调节和神经元过程中的多种作用。

Human

E. coli

N-10*His;N-SUMO;C-Myc

P26378-1 (M1-S380)

1996  [NCBI]

MVMIISTMEPQVSNGPTSNTSNGPSSNNRNCPSPMQTGATTDDSKTNLIVNYLPQNMTQEEFRSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVDQVTGVSRGVGFIRFDKRIEAEEAIKGLNGQKPSGATEPITVKFANNPSQKSSQALLSQLYQSPNRRYPGPLHHQAQRFRLDNLLNMAYGVKRLMSGPVPPSACPPRFSPITIDGMTSLVGMNIPGHTGTGWCIFVYNLSPDSDESVLWQLFGPFGAVNNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGDRVLQVSFKTNKAHKS

Approximately 61.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.