ENA-78/CXCL5 蛋白, Human

CXCL5, also known as neutrophil activating peptide 78 (ENA‐78), is a CXC chemokine containing ELR motif. CXCL5 promotes angiogenesis through interaction with its specific receptor CXCR2. CXCL5 is expressed by many immune cells, such as macrophages, eosinophils, and non-immune cells including mesothelial cells, and fibroblasts.CXCL5/CXCR2 axis not only contributes to the recruitment of neutrophils but also regulates the function of neutrophils in melanoma^[1][2]. ENA-78/CXCL5 Protein, Human is produced in E.coil, and consists of 70 amino acids (R45-N114).

CXCL5属于CXC型趋化因子家族，具有谷氨酸-亮氨酸-精氨酸的特定氨基酸序列，简称为ELR基序。CXC型趋化因子家族的ELR基序在血管生成中发挥重要作用。CXCL5 由许多免疫细胞表达，例如巨噬细胞、嗜酸性粒细胞，以及间皮细胞和成纤维细胞等非免疫细胞^[1][2]。
人CXCL5与小鼠和大鼠CXCL5有57%的氨基酸序列同一性。
CXCL5可以在免疫反应过程中将免疫系统中的T/B淋巴细胞和嗜酸性粒细胞等细胞募集到相应区域。此外，它还参与促进结缔组织的粘附和重塑。CXCL5在多种肿瘤组织中高表达。CXCL5可作为肿瘤预后的潜在指标。在肿瘤微环境（TME）中，CXCL5与其受体（例如CXCR2）结合，参与免疫细胞的募集并促进血管生成、肿瘤生长和转移。酸性TME或TNF-α激活的间充质干细胞（MSC）可以分泌CXCL5和其他促炎因子。此外，由纤溶酶原激活剂抑制剂-1（PAI-1）产生的癌症相关间皮细胞可以通过NF-κB信号通路分泌IL-8和CXCL5，从而促进腹膜转移。脂肪组织源性干细胞 (ASC) 分泌 CXCL5，随后促进肿瘤生长并影响乳腺肿瘤的发展^[2]。
CXCL5/CXCR2 轴招募中性粒细胞，促进血管生成和重塑结缔组织。此外，CXCL5 通过以 CXCR2 依赖性方式激活 AKT/NF-κB/FOXD1/VEGF-A 通路来促进血管生成。CXCL5 在癌细胞增殖、迁移和侵袭中发挥作用。CXCL5 促进多种肿瘤细胞的增殖，如前列腺癌、宫颈癌、肺癌、肝母细胞瘤和骨肉瘤中的肿瘤细胞^{[1][2] [3]}。

Recombinant human CXCL5 protein (1 ng/mL; 24-48 h) promotes proliferation, migration and partial invasion in Caco-2, HT-29 and SW-480 cells^[1].
Recombinant human CXCL5 protein (1-10 ng/mL; 36 h) stimulates the formation ability, proliferation, and migration in HUVECs, which are enhanced by the activation of the AKT/NF-κB/FOXD1/VEGF-A pathway in a CXCR2-dependent manner^[3].

1.The ED₅₀ is <50 ng/mL as measured by CHO-K1/Gα15/hCXCR2 cells (human Gα15 and human CXCR2 stably expressed in CHO-K1 cells).
2.Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 6.072 ng/mL, corresponding to a specific activity is 1.647×10⁵ U/mg.

• 
  Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 6.072 ng/mL, corresponding to a specific activity is 1.647×10⁵ U/mg.

Human

E. coli

Tag Free

P42830 (R45-N114)

6374  [NCBI]

RELRCVCLQTTQGVHPKMISNLQVFAIGPQCSKVEVVASLKNGKEICLDPEAPFLKKVIQKILDGGNKEN

Approximately 7.7 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Kawamura M, et al. CXCL5, a promoter of cell proliferation, migration and invasion, is a novel serum prognostic marker in patients with colorectal cancer. Eur J Cancer. 2012 Sep;48(14):2244-51.  [Content Brief]

  [2]. Kawamura M, et al. CXCL5, a promoter of cell proliferation, migration and invasion, is a novel serum prognostic marker in patients with colorectal cancer. Eur J Cancer. 2012 Sep;48(14):2244-51.  [Content Brief]

  [3]. Wen Zhang, et al. CXCL5/CXCR2 axis in tumor microenvironment as potential diagnostic biomarker and therapeutic target. Cancer Commun (Lond). 2020 Mar;40(2-3):69-80.  [Content Brief]