1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  3. Ephrin/Eph Family Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Eph Receptors
  5. EphA5
  6. EPHA5 蛋白, Human (HEK293, His)

EPHA5 蛋白, Human (HEK293, His)

目录号: HY-P77930
产品使用指南

EPHA5 蛋白是一种受体酪氨酸激酶,它与 GPI 锚定的肝配蛋白-A 配体混杂结合以启动双向信号传导。它参与正向和反向信号传导途径,EFNA5 可能作为其同源配体。EPHA5 蛋白, Human (HEK293, His) 是重组的 EPHA5 蛋白,由 HEK293 表达,带有 C-His 标签。

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描述

The EPHA5 protein is a receptor tyrosine kinase that promiscuously binds to the GPI-anchored ephrin-A ligand to initiate bidirectional signaling. It is involved in forward and reverse signaling pathways, and EFNA5 may serve as its cognate ligand. EPHA5 Protein, Human (HEK293, His) is the recombinant human-derived EPHA5 protein, expressed by HEK293 , with C-His labeled tag.

研究背景

EPHA5 蛋白是一种受体酪氨酸激酶,与邻近细胞上 GPI 锚定的肝配蛋白 A 家族配体混杂结合,启动接触依赖性双向信号传导。源自受体的下游途径称为正向信号传导,而肝配蛋白配体下游的途径称为反向信号传导。在 GPI 锚定的肝配蛋白-A 配体中,EFNA5 可能是 EPHA5 的同源/功能配体。EPHA5 在发育过程中充当轴突引导分子,可能参与形成视网膜顶盖、内鼻海马和海马间隔通路。它与 EFNA5 一起,通过调节突触发生,在成人大脑的突触可塑性中发挥作用。除了在神经系统中的功能之外,EPHA5 与 EFNA5 的相互作用还介导胰岛细胞之间的通讯,有助于调节葡萄糖刺激的胰岛素分泌。

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P54756-1 (A26-P573)

基因 ID
蛋白结构
N-term
EPHA5 (A26-P573)
Accession # P54756-1
His
C-term
同用名
EHK-1; EK7; BSK; EHK1; HEK7; TYRO4; EphA5; Rek7; TYRO4HEK7CEK7
分子量

68-80 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
EPHA5 Protein, Human (HEK293, His)
目录号:
HY-P77930
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