EphB2 蛋白, Human (HEK293, His-Fc)

EphB2 protein is a receptor tyrosine kinase that participates in bidirectional signaling with the transmembrane ephrin B ligand. It guides commissural axons in the developing cerebral cortex, efferent growth cones of the inner ear, and retinal ganglion cell axons. EphB2 Protein, Human (HEK293, His-Fc) is the recombinant human-derived EphB2 protein, expressed by HEK293 , with C-hFc, C-8*His labeled tag.

EphB2 蛋白是一种受体酪氨酸激酶，与邻近细胞上的跨膜肝配蛋白-B 配体相互作用，产生双向信号传导。EphB2 下游的通路称为正向信号传导，而肝配蛋白配体下游的通路称为反向信号传导。EphB2在发育过程中的轴突引导中发挥着至关重要的作用，特别是在连接大脑皮层颞叶的连合轴突的引导，以及内耳传出生长锥和视网膜神经节细胞轴突的引导中。它还调节树突棘的发育和成熟，促进兴奋性突触的形成，抵消 EFNB1 激活后 ARHGEF15 介导的负调节。此外，EphB2 参与各种发育过程，包括血管生成、上颚发育和内耳内淋巴生成。它与 EFNB2 的复合物控制尿道管状化和泄殖腔分隔过程中的细胞运动和粘附。此外，EphB2 可能具有肿瘤抑制活性，并可能影响血小板活化和凝血。

Immobilized Ephrin B2 Protein at 2 μg/mL (100 μL/well) can bind Biotinylated EphB2 Protein. The ED₅₀ for this effect is 1.949 ng/mL, corresponding to a specific activity is 5.13×105 Unit/mg.

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  Immobilized Ephrin B2 Protein at 2 μg/mL (100 μL/well) can bind  Biotinylated EphB2 Protein. The ED₅₀ for this effect is 1.949 ng/mL, corresponding to a specific activity is 5.13×10⁵ Unit/mg.

Human

HEK293

C-hFc;C-8*His

P29323-1/NP_059145.2 (V19-L543)

2048  [NCBI]

VEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRSGFYLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIQEKLPL

approximately 95 kDa

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  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.