1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Ephrin/Eph Family
  4. Ephrins
  5. Ephrin-A1
  6. Ephrin-A1/EFNA1 蛋白, Mouse (HEK293, Fc)

Ephrin-A1/EFNA1 蛋白, Mouse (HEK293, Fc)

目录号: HY-P75234
产品使用指南

Ephrin-A1/EFNA1 蛋白是一种 GPI 结合配体,对于发育中的神经元、血管和上皮细胞的迁移、排斥和粘附至关重要。它与附近的 Eph 受体结合,启动双向信号传导。Ephrin-A1/EFNA1 蛋白, Mouse (HEK293, Fc) 是重组的 Ephrin-A1/EFNA1 蛋白,由 HEK293 表达,带有 C-hFc 标签。Ephrin-A1/EFNA1 蛋白, Mouse (HEK293, Fc) 全长 164 个氨基酸,分子量约为 ~53 kDa。

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描述

The Ephrin-A1/EFNA1 protein is a GPI-binding ligand critical for migration, repulsion, and adhesion in developing neurons, blood vessels, and epithelia. It binds to nearby Eph receptors, initiating bidirectional signaling. Ephrin-A1/EFNA1 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Ephrin-A1/EFNA1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Ephrin-A1/EFNA1 Protein, Mouse (HEK293, Fc) is 164 a.a., with molecular weight of ~53 kDa.

研究背景

Ephrin-A1/EFNA1 蛋白是 Eph 受体的细胞表面 GPI 结合配体,在神经元、血管和上皮发育过程中的迁移、排斥和粘附中发挥关键作用。它与相邻细胞上的 Eph 受体混杂结合,引发接触依赖性双向信号传导。EFNA1 诱导的 RAC1 GTPase 激活和血管内皮细胞迁移在血管生成和肿瘤新生血管形成中至关重要,这取决于磷酸化 EPHA2 募集 VAV2、VAV3 和 PI3 激酶 p85 亚基。值得注意的是,EFNA1 通过诱导酪氨酸磷酸化激活和下调 EPHA2,从而导致内化和降解,从而发挥抗癌作用。在神经胶质瘤中,它充当负调节因子,下调 EPHA2FAK,从而发挥抑制肿瘤发生的作用。EFNA1可以诱导胚胎神经元生长锥的塌陷并调节树突棘形态发生。它以单体和同二聚体形式存在,与 EPHA2 形成异二聚体,并与一系列受体酪氨酸激酶结合,包括 EPHA1、EPHA3、EPHA4、EPHA5、EPHA6 和 EPHA7

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

P52793 (D19-S182)

基因 ID
蛋白结构
N-term
EFNA1 (D19-S182)
Accession # P52793
hFc
C-term
同用名
Ephrin-A1; LERK-1; EFNA1; EPLG1; TNFAIP4
分子量

Approximately 53 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Ephrin-A1/EFNA1 Protein, Mouse (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Ephrin-A1/EFNA1 Protein, Mouse (HEK293, Fc)
目录号:
HY-P75234
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