1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Ephrin/Eph Family
  4. Ephrins
  5. Ephrin-A5
  6. Ephrin-A5/EFNA5 蛋白, Mouse (HEK293, Fc)

Ephrin-A5/EFNA5 蛋白, Mouse (HEK293, Fc)

目录号: HY-P75232
产品使用指南

Ephrin-A5/EFNA5 蛋白是 Eph 受体的 GPI 结合配体,在神经元、血管和上皮发育中发挥着至关重要的作用。它与附近的 Eph 受体结合,启动双向信号传导并调节细胞间粘附和细胞骨架组织。Ephrin-A5/EFNA5 蛋白, Mouse (HEK293, Fc) 是重组的 Ephrin-A5/EFNA5 蛋白,由 HEK293 表达,带有 C-hFc 标签。Ephrin-A5/EFNA5 蛋白, Mouse (HEK293, Fc) 全长 183 个氨基酸,分子量约为 ~52 kDa。

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描述

Ephrin-A5/EFNA5 protein is the GPI-binding ligand of Eph receptor and plays a crucial role in neuronal, vascular and epithelial development. It binds to nearby Eph receptors, initiating bidirectional signaling and regulating intercellular adhesion and cytoskeletal organization. Ephrin-A5/EFNA5 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Ephrin-A5/EFNA5 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Ephrin-A5/EFNA5 Protein, Mouse (HEK293, Fc) is 183 a.a., with molecular weight of ~52 kDa.

研究背景

Ephrin-A5/EFNA5 蛋白是 Eph 受体的细胞表面 GPI 结合配体,在神经元、血管和上皮发育中发挥着关键作用,其中 Eph 受体对于迁移、排斥和粘附至关重要。EFNA5 与相邻细胞上的 Eph 受体混杂结合,启动接触依赖性双向信号传导,其中正向信号传导在受体下游,反向信号传导在肝配蛋白配体下游。当该蛋白与其同源受体的胞外域结合时,会在类似小窝的膜微域内诱导区室化信号传导,这一过程需要 Fyn 酪氨酸激酶的活性。EFNA5 激活 EPHA3 受体,调节细胞间粘附和细胞骨架组织,并且与 EPHA2 结合,可能有助于塑造晶状体纤维细胞并维持晶状体透明度。它可以主动刺激轴突束颤并介导胰岛细胞之间的通讯,影响葡萄糖刺激的胰岛素分泌。作为 EPHA7 的同源配体,EFNA5 通过调节细胞间的粘附和排斥来调节大脑发育。它与受体酪氨酸激酶 EPHA2、EPHA3 和 EPHB1 结合,并与 EPHA3ADAM10 形成三元复合物,通过 ADAM10 介导 EFNA5 胞外结构域脱落,进而调节 EFNA5-EPHA3 复合物的内化和功能。EFNA5 还与 EPHB2 结合并与 EPHA8 相互作用,激活后者受体。

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

O08543 (Q21-N203)

基因 ID
蛋白结构
N-term
EFNA5 (Q21-N203)
Accession # O08543
hFc
C-term
同用名
Ephrin-A5; AL-1; EPH-related receptor tyrosine kinase ligand 7; LERK-7; EFNA5; EPLG7
分子量

Approximately 52 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Ephrin-A5/EFNA5 Protein, Mouse (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Ephrin-A5/EFNA5 Protein, Mouse (HEK293, Fc)
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HY-P75232
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