ERO1L 蛋白, Human (HEK293, His)

ERO1L protein is an oxidoreductase that can effectively reoxidize P4HB/PDI in the endoplasmic reticulum and promote the formation of disulfide bonds. It transfers electrons to molecular oxygen, producing reactive oxygen species (ROS). ERO1L Protein, Human (HEK293, His) is the recombinant human-derived ERO1L protein, expressed by HEK293 , with C-6*His labeled tag. The total length of ERO1L Protein, Human (HEK293, His) is 445 a.a., with molecular weight of ~71.0 kDa.

ERO1L 蛋白充当氧化还原酶，促进内质网 (ER) 内二硫键的形成。其主要作用包括有效地再氧化 P4HB/PDI，这是一种对于催化蛋白质二硫键形成至关重要的酶，使 P4HB 能够参与连续几轮的二硫键形成。P4HB 再氧化后，ERO1L 通过其黄素腺嘌呤二核苷酸 (FAD) 辅因子将电子转移到分子氧，导致细胞内产生活性氧 (ROS)。该蛋白对于免疫球蛋白的正确折叠至关重要，并且通过激活肌醇 1,4,5-三磷酸受体 IP3R1，特别是以 CHOP 依赖性方式，在 ER 应激诱导的细胞凋亡中发挥重要作用。此外，ERO1L 参与霍乱弧菌感染期间未折叠霍乱毒素的释放，有助于毒素的逆转位。

Human

HEK293

C-6*His

Q96HE7 (E24-H468)

30001  [NCBI]

EEQPPETAAQRCFCQVSGYLDDCTCDVETIDRFNNYRLFPRLQKLLESDYFRYYKVNLKRPCPFWNDISQCGRRDCAVKPCQSDEVPDGIKSASYKYSEEANNLIEECEQAERLGAVDESLSEETQKAVLQWTKHDDSSDNFCEADDIQSPEAEYVDLLLNPERYTGYKGPDAWKIWNVIYEENCFKPQTIKRPLNPLASGQGTSEENTFYSWLEGLCVEKRAFYRLISGLHASINVHLSARYLLQETWLEKKWGHNITEFQQRFDGILTEGEGPRRLKNLYFLYLIELRALSKVLPFFERPDFQLFTGNKIQDEENKMLLLEILHEIKSFPLHFDENSFFAGDKKEAHKLKEDFRLHFRNISRIMDCVGCFKCRLWGKLQTQGLGTALKILFSEKLIANMPESGPSYEFHLTRQEIVSLFNAFGRISTSVKELENFRNLLQNIH

Approximately 71.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.