Erythropoietin/EPO 蛋白, Mouse (HEK293, His)

Erythropoietin (EPO) is essential for erythrocyte regulation, influencing proliferation, differentiation, and maintaining a balanced erythrocyte mass. Binding to its receptor, EPOR, induces dimerization and activates JAK2, initiating signaling pathways, including STAT1 and STAT3. These events are pivotal in orchestrating erythropoiesis and maintaining red blood cell homeostasis. Erythropoietin/EPO Protein, Mouse (HEK293, His) is the recombinant mouse-derived Erythropoietin/EPO protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Erythropoietin/EPO Protein, Mouse (HEK293, His) is 166 a.a., with molecular weight of 30-40 kDa.

促红细胞生成素 (EPO) 是一种对于调节红细胞增殖和分化以及维持平衡的循环红细胞质量至关重要的激素。EPO 与其受体 EPOR 结合后，触发 EPOR 二聚化并随后激活 JAK2，启动特定的下游信号传导途径，特别是涉及 STAT1 和 STAT3。这些分子事件在协调有助于红细胞生成和红细胞水平整体稳态的过程中发挥着关键作用。

The cell proliferation assay using TF 1 human erythroleukemic cells has an ED₅₀ value of 0.05-0.35 ng/mL.

Mouse

HEK293

C-6*His

Q0VED9 (A27-R192)

13856  [NCBI]

APPRLICDSRVLERYILEAKEAENVTMGCAEGPRLSENITVPDTKVNFYAWKRMEVEEQAIEVWQGLSLLSEAILQAQALLANSSQPPETLQLHIDKAISGLRSLTSLLRVLGAQKELMSPPDTTPPAPLRTLTVDTFCKLFRVYANFLRGKLKLYTGEVCRRGDR

30-40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.