1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Erythropoietin 蛋白, Rat (sf9, His)

Erythropoietin 蛋白, Rat (sf9, His)

目录号: HY-P73040
产品使用指南

Epo蛋白是一种激素,在调节体内红细胞生成方面发挥着至关重要的作用。它刺激骨髓中红细胞的分化和成熟。Erythropoietin 蛋白, Rat (sf9, His) 是重组的 促红细胞生成素蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。

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描述

Epo protein is a hormone that plays a vital role in regulating red blood cell production in the body.It stimulates the differentiation and maturation of red blood cells in the bone marrow.Erythropoietin Protein, Rat (sf9, His) is the recombinant rat-derived Erythropoietin protein, expressed by Sf9 insect cells , with C-His labeled tag.

研究背景

Epo蛋白是一种激素,主要负责调节红细胞的增殖和分化,以及维持循环红细胞质量的平衡水平。它通过与其受体 EPOR 结合来实现这一点,从而导致 EPOR 二聚化并随后激活 JAK2。这种激活会触发一系列涉及特定下游效应器(例如 STAT1 和 STAT3)的信号传导事件。这些途径,包括 RAS-MAPK 和 JAK-STAT5 途径,有助于 Epo 蛋白的多种功能。此外,Epo 蛋白以通过二硫键连接的同二聚体形式存在。

种属

Rat

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

P29676-1 (A27-R192)

基因 ID
蛋白结构
N-term
Erythropoietin (A27-R192)
Accession # P29676-1
His
C-term
同用名
ECYT5; EP; EPO; epoetin; Erythropoietin; MVCD2
氨基酸序列

MGVPERPTLLLLLSLLLIPLGLPVLCAPPRLICDSRVLERYILEAKEAENVTMGCAEGPRLSENITVPDTKVNFYAWKRMKVEEQAVEVWQGLSLLSEAILQAQALQANSSQPPESLQLHIDKAISGLRSLTSLLRVLGAQKELMSPPDATQAAPLRTLTADTFCKLFRVYSNFLRGKLKLYTGEACRRGDR

分子量

Approximately 27 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Erythropoietin Protein, Rat (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Erythropoietin Protein, Rat (sf9, His)
目录号:
HY-P73040
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