FABP3 蛋白, Human (His)

FABP3 Protein, Human (His) is a recombinant FABP3 protein with a His-flag. FABP3 Protein is expressed in the skeletal muscle, heart, brain and brown adipose tissue^[1].

脂肪酸结合蛋白 (FABP) 是细胞内蛋白，对小亲脂性配体表现出高亲和力。FABP-3 在骨骼肌、心脏、大脑和棕色脂肪组织中表达。食用高脂肪饮食后，其在骨骼肌中的表达会升高。其编码基因定位于人类染色体1p35。
FABP3是新近引入的急性心肌梗死(AMI)血浆标志物。FABP3 被确定为 OPTN 的新型选择性自噬底物（optineurin 是一种巨自噬/自噬受体，被发现在选择性自噬中发挥关键作用，将自噬与骨代谢耦合）。FABP3 促进脂肪生成并抑制 MSC 的成骨。敲除 FABP3 可减轻 optn-/- 小鼠和老年小鼠的骨质流失^[1][2]。

Human

E. coli

N-6*His

P05413 (V2-A133)

2170  [NCBI]

VDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTFKNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLILTLTHGTAVCTRTYEKEA

Approximately 15.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 6.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Seung-Min Lee, et al. FABP3-mediated membrane lipid saturation alters fluidity and induces ER stress in skeletal muscle with aging. Nat Commun. 2020 Nov 9;11(1):5661.  [Content Brief]

  [2]. Zheng-Zhao Liu, et al. Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3. Autophagy. 2020 Nov 4;1-17.  [Content Brief]