FAM20B 蛋白, Human (HEK293, Fc)

The FAM20B protein plays a key role in regulating mature glycosaminoglycan (GAG) chains by catalyzing xylose 2-O-phosphorylation in the glycosaminoglycan-protein linking region of the proteoglycan. This process is critical for the synthesis of sulfated GAGs (such as heparan sulfate and chondroitin sulfate) on the common GAG-protein linking region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of the core protein. FAM20B Protein, Human (HEK293, Fc) is the recombinant human-derived FAM20B protein, expressed by HEK293 , with N-hFc labeled tag. The total length of FAM20B Protein, Human (HEK293, Fc) is 379 a.a., with molecular weight of ~75-80 KDa.

FAM20B 蛋白通过催化蛋白聚糖糖胺聚糖-蛋白质连接区域内木糖的 2-O-磷酸化，在成熟糖胺聚糖 (GAG) 链的调节中发挥关键作用。该过程是核心蛋白共同 GAG-蛋白连接区 (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) 上硫酸化 GAG（包括硫酸乙酰肝素和硫酸软骨素）合成的组成部分。通过特定糖基转移酶逐步添加单糖残基形成该连接区域，其中木糖 2-O-磷酸化在 B3GAT3 (GlcAT-I) 的催化活性中发挥调节作用。B3GAT3 完成了 GAG-蛋白质连接区域的前体四糖，为后续重复二糖区域的合成奠定了基础。FAM20B 参与木糖 2-O-磷酸化，凸显了其对 GAG 链组装的精确调节和糖胺聚糖结构的复杂编排的重要贡献。

Measured by its ability to enhance survival of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 1.464 μg/mL. Corresponding to a specific activity is 683.06 unit/mg.

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  Measured by its ability to enhance survival of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 1.464 μg/mL. Corresponding to a specific activity is 683.06 Unit/mg.

Human

HEK293

N-hFc

O75063/NP_055679.1 (S31-L409)

9917  [NCBI]

SAANREDQRAFHRMMTGLRVELAPKLDHTLQSPWEIAAQWVVPREVYPEETPELGAVMHAMATKKIIKADVGYKGTQLKALLILEGGQKVVFKPKRYSRDHVVEGEPYAGYDRHNAEVAAFHLDRILGFHRAPLVVGRFVNLRTEIKPVATEQLLSTFLTVGNNTCFYGKCYYCRETEPACADGDIMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCDAVKKTSPYDSGPRLLDIIDTAVFDYLIGNADRHHYESFQDDEGASMLILLDNAKSFGNPSLDERSILAPLYQCCIIRVSTWNRLNYLKNGVLKSALKSAMAHDPISPVLSDPHLDAVDQRLLSVLATVKQCTDQFGMDTVLVEDRMPLSHL

Approximately 75-80 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.