FGFR-1 alpha (IIIc) 蛋白, Human (HEK293, His-Avi)

FGFR-1 alpha is a tyrosine-protein kinase receptor for fibroblast growth factor that is critical in embryonic development, cell proliferation, differentiation, and migration. GnRH is essential for mesodermal patterning, axial organization, skeletogenesis, and nervous system development. FGFR-1 alpha (IIIc) Protein, Human (HEK293, His-Avi) is the recombinant human-derived FGFR-1 alpha, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of FGFR-1 alpha (IIIc) Protein, Human (HEK293, His-Avi) is 353 a.a., with molecular weight of 65-85 kDa.

FGFR-1 α 是一种酪氨酸蛋白激酶，作为成纤维细胞生长因子的细胞表面受体，在调节胚胎发育、细胞增殖、分化和迁移中发挥着关键作用。它对于正常的中胚层模式、胚胎发育过程中的正确轴向组织、骨骼发生以及促性腺激素释放激素 (GnRH) 神经系统的发育至关重要。配体结合后，FGFR-1 α 激活多个信号级联，磷酸化 PLCG1、FRS2、GAB1 和 SHB 等关键蛋白。这种激活导致通过 PLCG1 产生信号分子，如二酰基甘油和肌醇 1,4,5-三磷酸。此外，FRS2 的磷酸化会触发 GRB2、GAB1、PIK3R1 和 SOS1 的募集，介导 RAS、MAPK1/ERK2、MAPK3/ERK1、MAP 激酶信号通路和 AKT1 信号通路的激活。FGFR-1 α 还促进 SHC1、STAT1 和 PTPN11/SHP2 的磷酸化。在细胞核内，它增强 RPS6KA1 和 CREB1 的活性，有助于转录调节。FGFR-1 α 信号传导的下调通过 IL17RD/SEF 和 FGFR-1 α 泛素化、内化和降解发生。

Immobilized Human FGFR1 alpha (IIIc) at 0.5 μg/mL (100 μl/Well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC₅₀ of ≤12.9 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

P11362-7 (R22-E374)

2260  [NCBI]

65-85 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.