1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens Receptor Proteins Enzymes & Regulators
  3. FGF Family Stem Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. FGFR-1 FGFR
  5. FGFR-1
  6. FGFR-1 蛋白, Mouse (HEK293, Fc)

FGFR-1 蛋白, Mouse (HEK293, Fc)

目录号: HY-P75767
产品使用指南

FGFR-1 蛋白是成纤维细胞生长因子的酪氨酸蛋白激酶受体,对胚胎发育、细胞增殖、分化和迁移至关重要。GnRH 对于中胚层模式、轴向组织、骨骼发生和神经系统发育至关重要。FGFR-1 蛋白, Mouse (HEK293, Fc) 是重组的 FGFR-1 蛋白,由 HEK293 表达,带有 C-hFc 标签。

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描述

The FGFR-1 protein is a tyrosine-protein kinase receptor for fibroblast growth factors that is critical in embryonic development, cell proliferation, differentiation, and migration. GnRH is essential for mesodermal patterning, axial organization, skeletogenesis, and nervous system development. FGFR-1 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived FGFR-1 protein, expressed by HEK293 , with C-hFc labeled tag.

研究背景

FGFR-1 蛋白是一种酪氨酸蛋白激酶,作为成纤维细胞生长因子的细胞表面受体,在胚胎发育、细胞增殖、分化和迁移的复杂调节中发挥着至关重要的作用。它对于正常中胚层模式、胚胎发育过程中正确的轴向组织、骨骼发生以及促性腺激素释放激素 (GnRH) 神经系统的发育是不可或缺的。配体结合后,FGFR-1 激活多个信号级联,磷酸化 PLCG1、FRS2、GAB1 和 SHB 等关键蛋白。这种激活导致通过 PLCG1 产生信号分子,如二酰基甘油和肌醇 1,4,5-三磷酸。此外,FRS2 的磷酸化会触发 GRB2、GAB1、PIK3R1 和 SOS1 的募集,介导 RAS、MAPK1/ERK2、MAPK3/ERK1、MAP 激酶信号通路和 AKT1 信号通路的激活。FGFR1 还促进 SHC1、STAT1 和 PTPN11/SHP2 的磷酸化。在细胞核内,它增强 RPS6KA1 和 CREB1 的活性,有助于转录调节。FGFR1 信号传导的下调通过 IL17RD/SEF 和 FGFR1 泛素化、内化和降解发生。

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

P16092 (R22-E376)

基因 ID
蛋白结构
N-term
FGFR-1 (R22-E376)
Accession # P16092
hFc
C-term
同用名
Fibroblast growth factor receptor 1; FGFR-1; BFGFR; FLT-2; CD331; HBGFR
分子量

100-110 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FGFR-1 Protein, Mouse (HEK293, Fc)
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