FGFR-3 蛋白, Mouse (HEK293, His)

The FGFR-3 protein is a tyrosine protein kinase that serves as a cell surface receptor for fibroblast growth factors and controls cellular processes, including proliferation, differentiation, and apoptosis. It is essential for chondrocyte differentiation, proliferation, apoptosis and normal bone development functions. FGFR-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FGFR-3 protein, expressed by HEK293 , with C-His labeled tag. The total length of FGFR-3 Protein, Mouse (HEK293, His) is 347 a.a., with molecular weight of 60-90 kDa.

FGFR-3 蛋白是一种酪氨酸蛋白激酶，作为成纤维细胞生长因子的细胞表面受体，对于调节细胞增殖、分化和凋亡至关重要。它在软骨细胞分化、增殖和凋亡以及正常骨骼发育中起着至关重要的作用。FGFR-3 还调节成骨细胞中的成骨和出生后骨矿化。它可以促进软骨细胞凋亡，但也与癌细胞增殖有关。此外，FGFR-3 对于内耳的发育至关重要，并磷酸化 PLCG1、CBL 和 FRS2。配体与 FGFR-3 的结合会触发各种信号级联反应，包括通过激活 PLCG1 产生二酰基甘油和肌醇 1,4,5-三磷酸。FRS2 的磷酸化导致 GRB2、GAB1、PIK3R1 和 SOS1 的募集，最终激活 RAS、MAPK1/ERK2、MAPK3/ERK1 和 AKT1 信号通路。FGFR-3 还在维生素 D 代谢调节中发挥作用。当突变导致组成型激酶激活或损害正常的 FGFR3 成熟、内化和降解时，就会发生异常信号传导。FGFR-3 的过表达或组成型激活可促进 STAT1、STAT5A 和 STAT5B 的激活。此外，FGFR-3 参与出生后肺部发育。

Immobilized FGF basic at 5 µg/mL (100 µL/well) can bind Biotinylated FGFR-3. The ED₅₀ for this effect is 50.77 ng/mL.

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  Immobilized FGF basic at 5 µg/mL (100 µL/well) can bind Biotinylated FGF R3. The ED₅₀ for this effect is 50.77 ng/mL.

Mouse

HEK293

C-His

Q61851 (E21-Y367)

14184  [NCBI]

EPPGPEQRVVRRAAEVPGPEPSQQEQVAFGSGDTVELSCHPPGGAPTGPTVWAKDGTGLVASHRILVGPQRLQVLNASHEDAGVYSCQHRLTRRVLCHFSVRVTDAPSSGDDEDGEDVAEDTGAPYWTRPERMDKKLLAVPAANTVRFRCPAAGNPTPSISWLKNGKEFRGEHRIGGIKLRHQQWSLVMESVVPSDRGNYTCVVENKFGSIRQTYTLDVLERSPHRPILQAGLPANQTAILGSDVEFHCKVYSDAQPHIQWLKHVEVNGSKVGPDGTPYVTVLKTAGANTTDKELEVLSLHNVTFEDAGEYTCLAGNSIGFSHHSAWLVVLPAEEELMETDEAGSVY

60-90 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.