FHIT 蛋白, Human (His)

FHIT protein has multiple enzymatic activities, including dinucleoside triphosphohydrolase, adenylate sulfatase and adenosine 5'-monophosphate amidase, and functions as a tumor suppressor. It regulates CTNNB1-mediated transcriptional activation, affecting key genes such as CCND1 and BIRC5. FHIT Protein, Human (His) is the recombinant human-derived FHIT protein, expressed by E. coli , with C-His labeled tag. The total length of FHIT Protein, Human (His) is 147 a.a., with molecular weight of ~18 kDa.

FHIT 蛋白具有二核苷三磷酸水解酶活性，可特异性裂解 P(1)-P(3)-双(5'-腺苷基)三磷酸 (Ap3A)，生成 AMP 和 ADP。此外，它还具有腺苷硫酸酯酶活性，可将腺苷 5'-磷酸硫酸盐水解为 AMP 和硫酸盐，还具有腺苷 5'-单磷酸酰胺酶活性，可分解嘌呤核苷酸氨基磷酸酯，如腺苷 5'-单磷酸酰胺 (AMP-NH2)，产生 AMP 和 NH2。作为肿瘤抑制因子，FHIT 通过 CTNNB1 调节转录激活，影响与细胞增殖和存活相关的关键基因的表达，例如 CCND1 和 BIRC5。此外，FHIT 通过 SRC 和 AKT1 信号通路参与细胞凋亡诱导，抑制 MDM2 介导的 p53/TP53 蛋白酶体降解，并有助于 p53/TP53 介导的细胞凋亡。细胞凋亡效应可能涉及FHIT 结合Ap3A 或相关化合物的能力，并且部分归因于其线粒体形式，该形式增强线粒体钙摄取并使低亲和力Ca(2+) 转运蛋白敏感。

Measured by its ability to inhibit the proliferation of A549 cells. The ED₅₀ for this effect is 0.1098 ng/mL, corresponding to a specific activity is 9.107×10⁶ units/mg.

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  Measured by its ability to inhibit the proliferation of A549 cells.The ED₅₀ for this effect is 0.1098 ng/mL , corresponding to a specific activity is 9.107×10⁶ units/mg.

Human

E. coli

C-6*His

P49789 (M1-Q147)

2272  [NCBI]

MSFRFGQHLIKPSVVFLKTELSFALVNRKPVVPGHVLVCPLRPVERFHDLRPDEVADLFQTTQRVGTVVEKHFHGTSLTFSMQDGPEAGQTVKHVHVHVLPRKAGDFHRNDSIYEELQKHDKEDFPASWRSEEEMAAEAAALRVYFQ

Approximately 18 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping with dry ice.