FLRT3 蛋白, Human (HEK293, His)

The FLRT3 protein exhibits multiple roles in cellular processes, affecting cell-cell adhesion, migration, and axonal guidance, with context-dependent attraction or repulsion.FLRT3 is critical for the spatial organization of brain neurons and also contributes to retinal blood vessel development.FLRT3 Protein, Human (HEK293, His) is the recombinant human-derived FLRT3 protein, expressed by HEK293 , with C-6*His labeled tag.

FLRT3 蛋白在细胞过程中发挥多方面的作用，例如细胞间粘附、细胞迁移和轴突引导，表现出吸引力或排斥作用取决于其相互作用伙伴。FLRT3 对大脑神经元的空间组织至关重要，还有助于视网膜血管的发育。FLRT3 通过与 ADGRL3 以及相邻细胞上潜在的其他懒虫蛋白相互作用，参与细胞间粘附。它与 ROBO1 细胞内结构域的相互作用介导轴突对表达 NTN1 的细胞的吸引力，同时还促进轴突生长锥塌陷，并通过 UNC5B 和潜在的其他 UNC-5 家族成员在神经元引导中发挥排斥作用。FLRT3 促进神经突生长并介导细胞间接触，从而增加神经突数量和长度。此外，它还调节谷氨酸能突触的密度，并参与成纤维细胞生长因子介导的信号级联反应。FLRT3 对于胚胎发育过程中的正常形态发生至关重要，有助于腹侧闭合、头褶融合和定形内胚层迁移等过程，以及正常基膜和前内脏内胚层的形成和维持。FLRT3 形成单体、同源二聚体，并通过富含亮氨酸的重复序列自缔合，形成同源寡聚体。相互作用包括与 FGFR1、ADGRL1/LPHN1、LPHN2、ADGRL3、UNC5B、UNC5D 和 ROBO1 的相互作用。

Human

HEK293

C-6*His

Q9NZU0 (K29-P528)

23767  [NCBI]

KSCPSVCRCDAGFIYCNDRFLTSIPTGIPEDATTLYLQNNQINNAGIPSDLKNLLKVERIYLYHNSLDEFPTNLPKYVKELHLQENNIRTITYDSLSKIPYLEELHLDDNSVSAVSIEEGAFRDSNYLRLLFLSRNHLSTIPWGLPRTIEELRLDDNRISTISSPSLQGLTSLKRLVLDGNLLNNHGLGDKVFFNLVNLTELSLVRNSLTAAPVNLPGTNLRKLYLQDNHINRVPPNAFSYLRQLYRLDMSNNNLSNLPQGIFDDLDNITQLILRNNPWYCGCKMKWVRDWLQSLPVKVNVRGLMCQAPEKVRGMAIKDLNAELFDCKDSGIVSTIQITTAIPNTVYPAQGQWPAPVTKQPDIKNPKLTKDHQTTGSPSRKTITITVKSVTSDTIHISWKLALPMTALRLSWLKLGHSPAFGSITETIVTGERSEYLVTALEPDSPYKVCMVPMETSNLYLFDETPVCIETETAPLRMYNPTTTLNREQEKEPYKNPNLP

60-110 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.