FOLR1 蛋白, Human (HEK293, His)

The FOLR1 protein is an important mediator of folate uptake, binding to folate and promoting the delivery of 5-methyltetrahydrofolate into cells. Studies show high affinity at neutral pH. FOLR1 Protein, Human (HEK293, His) is the recombinant human-derived FOLR1 protein, expressed by HEK293 , with C-His labeled tag.

FOLR1 蛋白作为叶酸摄取的关键介质，与叶酸和还原叶酸衍生物结合，促进 5-甲基四氢叶酸和叶酸类似物输送到细胞内部。各种研究证明，该过程的特点是在中性 pH 值下对叶酸和叶酸类似物具有高亲和力。值得注意的是，受体内吞作用后暴露于弱酸性 pH 值会引起构象变化，显着降低其对叶酸的亲和力，促进其释放。除了在叶酸转运中的作用之外，FOLR1 对于正常胚胎发育和适当的细胞增殖至关重要，强调了其在基本细胞过程中的重要性。

Measured by its binding ability in a functional ELISA. Immobilized FOLR1 at 1 μg/mL can bind Anti-FOLR1 antibody, Mouse IgG1, the EC₅₀ of human FOLR1 protein is ≤9.719 ng/mL, corresponding to a specific activity is ≥1.029×10⁵ units/mg.

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  Measured by its binding ability in a functional ELISA. Immobilized FOLR1 at 1 μg/ml can bind Anti-FOLR1 antibody, Mouse IgG1, the EC₅₀ of human FOLR1 protein is 7.461 ng/mL, corresponding to a specific activity is 1.340×10⁵ units/mg.

Human

HEK293

C-His

P15328 (R25-S234)

2348  [NCBI]

RIAWARTELLNVCMNAKHHKEKPGPEDKLHEQCRPWRKNACCSTNTSQEAHKDVSYLYRFNWNHCGEMAPACKRHFIQDTCLYECSPNLGPWIQQVDQSWRKERVLNVPLCKEDCEQWWEDCRTSYTCKSNWHKGWNWTSGFNKCAVGAACQPFHFYFPTPTVLCNEIWTHSYKVSNYSRGSGRCIQMWFDPAQGNPNEEVARFYAAAMS

34-38 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.