Fractalkine/CX3CL1 蛋白, Mouse (HEK293, His)

The Fractalkine/CX3CL1 protein is a multifunctional chemokine that binds to CX3CR1 and the integrins ITGAV:ITGB3 and ITGA4:ITGB1.It regulates immune responses, inflammation, cell adhesion, and chemotaxis.Fractalkine/CX3CL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Fractalkine/CX3CL1 protein, expressed by HEK293 , with C-6*His labeled tag.

Fractalkine/CX3CL1 蛋白是一种多功能趋化因子，可作为 CX3CR1 和整合素 ITGAV:ITGB3 和 ITGA4:ITGB1 的配体。CX3CR1-CX3CL1信号轴在不同组织区室中表现出不同的功能，包括免疫反应调节、炎症调节、细胞粘附和趋化性。在内皮相互作用的背景下，Fractalkine/CX3CL1 在调节白细胞粘附和迁移过程中发挥着关键作用。值得注意的是，它可以以 CX3CR1 依赖或 CX3CR1 独立的方式激活整合素，结合到整合素上的经典配体结合位点（位点 1）和不同位点（位点 2）。在存在 CX3CR1 的情况下，它直接通过位点 1 激活整合素，而在没有 CX3CR1 的情况下，它与位点 2 结合，从而增强其他整合素配体与位点 1 的结合。此外，Fractalkine/CX3CL1 的可溶形式表现出趋化特性适用于 T 细胞和单核细胞，但不适用于中性粒细胞。

Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ this effect is 9.640 ng/mL, corresponding to a specific activity is 103734.440 U/mg.

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  Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 9.640 ng/mL, corresponding to a specific activity is 103734.440 U/mg.

Mouse

HEK293

C-6*His

O35188 (Q25-R337)

20312  [NCBI]

QHLGMTKCEIMCDKMTSRIPVALLIRYQLNQESCGKRAIVLETTQHRRFCADPKEKWVQDAMKHLDHQAAALTKNGGKFEKRVDNVTPGITLATRGLSPSALTKPESATLEDLALELTTISQEARGTMGTSQEPPAAVTGSSLSTSEAQDAGLTAKPQSIGSFEAADISTTVWPSPAVYQSGSSSWAEEKATESPSTTAPSPQVSTTSPSTPEENVGSEGQPPWVQGQDLSPEKSLGSEEINPVHTDNFQERGPGNTVHPSVAPISSEETPSPELVASGSQAPKIEEPIHATADPQKLSVLITPVPDTQAATR

Approximately 55-85 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.